| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ubiquitin carboxyl-terminal hydrolase 45, Deubiquitinating enzyme 45, Ubiquitin thioesterase 45, Ubiquitin-specific-processing protease 45, USP45 |
| Entrez GeneID | 85015 |
| WB Predicted band size | 91.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This USP45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the Central region of human USP45. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于USP45抗体的3篇示例性参考文献(注：内容为假设性概括，实际文献可能需要根据具体研究补充或调整)：
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1. **文献名称**：*USP45 regulates DNA damage response through deubiquitinating BRCA1*
**作者**：Chen L, et al.
**摘要**：本研究利用特异性USP45抗体，通过免疫共沉淀和质谱分析，发现USP45与BRCA1相互作用，并调控其去泛素化修饰，从而影响DNA损伤修复过程。Western blot和免疫荧光实验验证了抗体在检测内源性USP45中的高特异性。
2. **文献名称**：*Development and validation of a monoclonal antibody against human USP45 for cancer biomarker studies*
**作者**：Wang Y, et al.
**摘要**：研究团队开发了一种针对人源USP45的单克隆抗体，验证了其在ELISA、免疫组化和流式细胞术中的应用。该抗体成功用于检测多种癌症组织中USP45的异常高表达，提示其作为潜在肿瘤标志物的可能性。
3. **文献名称**：*USP45 modulates neuronal apoptosis via p53 signaling: Evidence from antibody-based knockdown assays*
**作者**：Kim S, et al.
**摘要**：通过使用USP45抗体进行免疫沉淀和功能阻断实验，研究发现USP45通过去泛素化p53调控神经元凋亡。抗体特异性在小鼠脑组织中得到验证，为神经退行性疾病机制提供了新见解。
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如需查找真实文献，建议在PubMed或Google Scholar中检索关键词“USP45 antibody”或联系抗体供应商获取相关验证数据。
The USP45 antibody is a research tool designed to detect and study ubiquitin-specific protease 45 (USP45), a member of the deubiquitinating enzyme (DUB) family. USP45 is implicated in the removal of ubiquitin moieties from substrate proteins, thereby regulating their stability, localization, or activity. While less characterized than other DUBs, USP45 is suggested to play roles in DNA repair, cell cycle regulation, and cellular stress responses. Its potential interaction with proteins involved in genome maintenance (e.g., BRCA1/2 complexes) highlights its possible relevance in cancer biology.
USP45 antibodies are typically developed as monoclonal or polyclonal reagents, validated for applications like Western blotting, immunohistochemistry, and immunofluorescence to assess USP45 expression levels and subcellular localization. These antibodies aid in exploring USP45's tissue-specific distribution, overexpression in certain tumors, or dysregulation in neurological disorders. Studies using USP45 antibodies may also clarify its molecular mechanisms, such as binding partners or post-translational modifications. Despite limited functional data, USP45's structural homology to other USP family members suggests conserved catalytic activity, making it a target for therapeutic development, particularly in diseases linked to aberrant protein degradation. However, further research is required to fully define its biological roles and clinical significance.
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