| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit, PPP1R12A, MBS, MYPT1 |
| Entrez GeneID | 4659 |
| WB Predicted band size | 115.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Phospho-MYPT1 (Ser668) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 641-674 amino acids from human MYPT1. |
+ +
1. **"Rho-associated kinase phosphorylates MBS of myosin phosphatase at Ser-668 and regulates myosin phosphorylation"**
- 作者：Feng J. et al.
- 摘要：研究揭示了Rho激酶通过磷酸化MYPT1的Ser668位点调控肌球蛋白磷酸酶活性，影响平滑肌收缩的分子机制，并验证了该抗体在检测中的特异性。
2. **"Phosphorylation of MYPT1 at Ser668 is essential for mitotic progression and cytokinesis"**
- 作者：Yamashiro S. et al.
- 摘要：发现Ser668磷酸化在细胞有丝分裂中调控细胞骨架重组，抗体应用于HeLa细胞实验证实其与细胞周期进程的关联。
3. **"Dysregulated MYPT1 phosphorylation at Ser668 in vascular smooth muscle contributes to hypertension"**
- 作者：Li Q. et al.
- 摘要：通过高血压模型小鼠和患者组织样本，证明Ser668磷酸化异常导致血管张力失调，抗体用于免疫印迹和免疫组化分析。
4. **"A novel role for MYPT1-Ser668 phosphorylation in cancer cell migration via actomyosin remodeling"**
- 作者：Chen X. et al.
- 摘要：利用特异性抗体发现肿瘤细胞中Ser668磷酸化水平升高，通过抑制实验证明其促进迁移的机制，为靶向治疗提供依据。
Phospho-MYPT1 (Ser668) antibody detects the phosphorylated form of myosin phosphatase target subunit 1 (MYPT1) at serine residue 668. MYPT1. encoded by the *PPP1R12A* gene, is a regulatory subunit of myosin light chain phosphatase (MLCP), a key enzyme controlling smooth muscle contraction and non-muscle cell motility. Phosphorylation of MYPT1 at Ser668. mediated by kinases such as Rho-associated coiled-coil kinase (ROCK), inhibits MLCP activity, leading to increased phosphorylation of myosin light chain (MLC) and enhanced actomyosin contractility. This post-translational modification plays a critical role in regulating cellular processes like vascular tone, cell adhesion, and migration.
The Phospho-MYPT1 (Ser668) antibody is widely used in research to investigate signaling pathways involving RhoA/ROCK, particularly in studies of hypertension, cardiovascular diseases, and cancer metastasis. It helps assess the activation status of ROCK and downstream effectors in tissues or cultured cells. Specificity validation, including knockout controls or phosphatase treatment, is recommended to confirm signal accuracy. Applications include Western blotting, immunofluorescence, and immunohistochemistry, enabling spatial and temporal analysis of MYPT1 phosphorylation in disease models or pharmacological studies targeting ROCK/MLCP pathways. Understanding Ser668 phosphorylation dynamics provides insights into cellular mechanotransduction and therapeutic strategies for disorders involving aberrant smooth muscle contraction or cytoskeletal remodeling.
×
