Rabbit Polyclonal TOMM40(N-Term) Antibody

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     All lanes : Anti-TOMM40 Antibody (N-Term) at 1:2000 dilution Lane 1: Human brain lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: Hut78 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: A431 whole cell lysate Lane 7: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling TOMM40 with P34437 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing HeLa cells stained with P34437(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34437,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The TOMM40 (N-Term) antibody targets the N-terminal region of the Translocase of Outer Mitochondrial Membrane 40 (TOMM40), a key component of the mitochondrial protein import machinery. TOMM40 is essential for forming the TOM complex, which mediates the translocation of nuclear-encoded proteins across the mitochondrial outer membrane. This β-barrel channel protein plays a critical role in maintaining mitochondrial function, including energy production, apoptosis regulation, and cellular stress responses. Dysregulation of TOMM40 has been linked to neurodegenerative diseases, such as Alzheimer's disease (AD), due to its proximity to the APOE gene on chromosome 19 and potential involvement in lipid metabolism and neuronal health.

The TOMM40 (N-Term) antibody is widely used in research to investigate mitochondrial dynamics, protein import mechanisms, and disease-associated mitochondrial dysfunction. It enables the detection of TOMM40 expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping to assess mitochondrial integrity in cellular or tissue samples. Studies leveraging this antibody have explored its role in aging, neurodegeneration, and metabolic disorders, providing insights into how mitochondrial transport defects contribute to pathological conditions. Its specificity for the N-terminal region ensures recognition of full-length TOMM40. aiding in distinguishing functional protein from potential cleavage products or isoforms.