Rabbit Polyclonal NSFL1C Antibody

+ +

•  

   
     All lanes : Anti-NSFL1C Antibody (Center) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: NCI-H1299 whole cell lysate Lane 4: U-251 MG whole cell lysate Lane 5: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     Overlay histogram showing A431 cells stained with P34417(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min.  The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

•  

   
     P34417 staining NSFL1C in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The NSFL1C (NSFL1 cofactor, also known as p47 or UBXN2B) is a conserved protein implicated in ubiquitin-dependent cellular processes. It belongs to the UBXD family, characterized by ubiquitin-regulatory X (UBX) domains that facilitate interactions with valosin-containing protein (VCP/p97), a key ATPase involved in endoplasmic reticulum-associated degradation (ERAD), autophagy, and mitotic regulation. NSFL1C acts as a cofactor for p97. assisting in the disassembly of ubiquitinated protein complexes and substrate processing. Studies highlight its role in maintaining proteostasis, lipid droplet formation, and cell cycle control.

NSFL1C antibodies are essential tools for studying its expression, localization, and molecular interactions. They enable detection via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of NSFL1C is linked to pathological conditions, including cancers (e.g., breast, prostate) and neurodegenerative diseases, where disrupted protein degradation pathways contribute to pathogenesis. Elevated NSFL1C levels in tumors correlate with aggressive phenotypes, suggesting potential as a biomarker or therapeutic target. Conversely, reduced expression may impair ERAD, leading to toxic protein aggregation in neurodegeneration.

Research using NSFL1C antibodies continues to unravel its multifaceted roles in cellular stress responses, apoptosis, and DNA repair, offering insights into disease mechanisms and opportunities for targeted interventions.