Rabbit Polyclonal RARRES2(N-Term) Antibody

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     Anti-RARRES2 Antibody (N-Term) at 1:2000 dilution + Human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling RARRES2 with P34416 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG  secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing HepG2 cells stained with P34416(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34416,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The RARRES2 (N-Term) antibody is designed to target the N-terminal region of the Retinoic Acid Receptor Responder 2 (RARRES2) protein, also known as Chemerin. RARRES2 is a multifunctional adipokine and chemokine encoded by the *RARRES2* gene, involved in adipocyte differentiation, immune regulation, and inflammation. It exists as a precursor protein that undergoes proteolytic processing to generate bioactive isoforms, with the N-terminal fragment playing a critical role in binding to chemokine-like receptor 1 (CMKLR1) to mediate cellular signaling.

This antibody is widely used in research to study RARRES2 expression, localization, and function in metabolic disorders (e.g., obesity, diabetes), inflammatory diseases, and cancer. Its specificity for the N-terminal region allows detection of both precursor and processed forms of the protein in applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Studies have highlighted RARRES2's dual role in promoting adipogenesis while exerting anti-inflammatory or pro-tumorigenic effects depending on cellular context. The antibody's utility in characterizing RARRES2 dynamics in tissues, serum, or cell models makes it a valuable tool for exploring its therapeutic potential in metabolic and immune-related pathologies. Validation typically includes testing in knockout controls or recombinant protein assays to confirm target specificity.