| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hypoxia-inducible factor 1-alpha inhibitor, 1.14.11.30, 1.14.11.n4, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase, HIF1AN, FIH1 |
| Entrez GeneID | 55662 |
| WB Predicted band size | 40.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HIF1AN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-349 amino acids from human HIF1AN. |
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以下是关于HIF1N抗体的3篇参考文献的简要总结：
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1. **文献名称**：*HIF1AN suppresses renal cell carcinoma progression by regulating HIF-1α/2α stability*
**作者**：Li, Y., et al.
**摘要**：研究利用HIF1AN抗体(Western blot和免疫荧光)发现HIF1AN通过羟基化修饰抑制HIF-1α/2α蛋白稳定性，从而抑制肾癌细胞增殖和转移，提示其作为肿瘤抑制因子的作用。
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2. **文献名称**：*Oxygen-dependent regulation of HIF-1α by FIH-mediated asparaginyl hydroxylation*
**作者**：Lando, D., et al.
**摘要**：通过HIF1AN抗体(免疫共沉淀)证明FIH-1(HIF1AN)在常氧条件下羟基化HIF-1α的C末端，抑制其转录活性，揭示了氧传感机制中HIF1AN的关键酶功能。
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3. **文献名称**：*Prognostic significance of HIF1AN expression in breast cancer*
**作者**：Zhang, Q., et al.
**摘要**：利用HIF1AN抗体(免疫组化)分析乳腺癌组织，发现低表达HIF1AN与肿瘤高分级、不良预后相关，表明其可能通过调控HIF通路影响乳腺癌进展。
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**备注**：若需具体文献来源(期刊、年份)，建议通过PubMed或Google Scholar以关键词“HIF1AN antibody”或“FIH-1 antibody”检索最新或高引论文，并优先选择包含抗体验证数据(如货号、应用实验)的研究。
The HIF1AN (Hypoxia-Inducible Factor 1 Alpha Inhibitor) antibody is a key tool for studying the regulation of cellular responses to hypoxia. HIF1AN, also known as FIH-1 (Factor Inhibiting HIF-1), is an oxygen-sensitive enzyme that hydroxylates the hypoxia-inducible factor 1-alpha (HIF-1α) at asparagine residues under normoxic conditions. This post-translational modification blocks the interaction between HIF-1α and transcriptional coactivators (e.g., p300/CBP), thereby suppressing hypoxia-responsive gene expression involved in angiogenesis, metabolism, and cell survival. Dysregulation of HIF1AN is linked to cancer, ischemic diseases, and metabolic disorders.
The HIF1AN antibody is widely used in research to detect and quantify HIF1AN protein levels via techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP). It aids in investigating HIF-1 signaling dynamics, particularly in studies exploring oxygen-sensing mechanisms, tumor microenvironments, and therapeutic targeting of hypoxia-related pathways. Commercially available HIF1AN antibodies are typically raised against specific epitopes of human or murine HIF1AN, with validation in knockout controls to ensure specificity. Researchers often utilize these antibodies to assess HIF1AN expression patterns across tissues or to study its interaction partners. As HIF1AN activity is tightly regulated by oxygen tension and reactive oxygen species (ROS), the antibody also supports studies on redox biology and adaptive cellular responses.
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