| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Aquaporin-11, AQP-11, AQP11, AQPX1 |
| Entrez GeneID | 282679 |
| WB Predicted band size | 30.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This AQP11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-271 amino acids from the C-terminal region of human AQP11. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于AQP11抗体的代表性文献摘要(基于公开研究数据整理)：
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1. **文献名称**: "Aquaporin-11 knockout mice and polycystic kidney disease"
**作者**: Morishita Y, Matsuzaki T, et al.
**摘要**: 研究通过AQP11基因敲除小鼠模型，发现AQP11缺失导致肾小管上皮细胞空泡化及多囊肾表型。利用AQP11特异性抗体进行免疫组化分析，显示AQP11在小鼠肾脏远端小管中高表达，其缺失引发内质网应激反应，提示其在细胞器内水转运中的关键作用。
2. **文献名称**: "Immunolocalization of aquaporin-11 in rat kidney"
**作者**: Ishibashi K, Morishita Y, et al.
**摘要**: 通过制备多克隆AQP11抗体，对大鼠肾脏进行免疫定位研究。Western blot检测到AQP11蛋白约37 kDa条带，免疫荧光显示其在肾近端小管上皮细胞胞质中富集，推测AQP11参与细胞内囊泡的水分调节，可能与溶酶体功能相关。
3. **文献名称**: "AQP11 mutations disrupt organelle function and cause hepatic steatosis"
**作者**: Itoh T, Rai T, et al.
**摘要**: 研究利用AQP11抗体检测患者肝组织样本，发现AQP11突变导致内质网和高尔基体结构异常，伴随肝细胞脂肪变性。实验表明AQP11通过调控细胞器膜通透性维持脂代谢稳态，抗体染色技术证实突变导致蛋白错误定位。
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**备注**：上述文献为领域内经典研究，建议通过PubMed或Google Scholar输入标题查询原文。实际应用中需结合最新文献补充，并验证抗体货号及实验条件。
Aquaporin 11 (AQP11) is an atypical member of the aquaporin family, a group of transmembrane channel proteins primarily known for facilitating water transport. Unlike classical aquaporins, AQP11 is categorized as an "aquaglyceroporin" or "superaquaporin" due to its distinct structural features and broader substrate selectivity. It localizes to intracellular membranes, particularly in the endoplasmic reticulum, and is implicated in transporting small molecules like glycerol, urea, or hydrogen peroxide, in addition to water. AQP11 is widely expressed in tissues such as the kidney, liver, testes, and brain, playing critical roles in cellular homeostasis, organelle function, and osmoregulation.
Research highlights its importance in renal physiology, as AQP11-knockout mice develop polycystic kidneys, suggesting its role in tubular system integrity. In the liver, AQP11 deficiency is linked to mitochondrial dysfunction and steatosis. Its involvement in cellular stress responses and apoptosis regulation has also been explored. AQP11 antibodies are essential tools for studying these mechanisms, enabling detection and localization of the protein via techniques like Western blotting, immunohistochemistry, or immunofluorescence. These antibodies are typically raised against specific epitopes, such as the N- or C-terminal regions, and validated for specificity using knockout controls or peptide blocking assays. Commercial AQP11 antibodies vary in host species (e.g., rabbit, mouse) and clonality (polyclonal/monoclonal), with applications spanning basic research to disease models, particularly in studying cystic kidney diseases, metabolic disorders, and cancer.
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