| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase STK11, 2.7.11.1, Liver kinase B1, LKB1, hLKB1, Renal carcinoma antigen NY-REN-19, STK11, LKB1, PJS |
| Entrez GeneID | 6794 |
| WB Predicted band size | 48.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Phospho-LKB1 (Ser428) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 398-429 amino acids from human LKB1. |
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以下是关于Phospho-LKB1 (Ser428)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"LKB1 is a master kinase that activates AMPK and other AMPK-related kinases"*
**作者**：Lizcano, J.M. et al.
**摘要**：该研究阐明了LKB1通过磷酸化AMPK家族激酶调控细胞能量代谢的机制，并利用Phospho-LKB1 (Ser428)抗体证实了该位点的磷酸化对LKB1激酶活性的必要性。
2. **文献名称**：*"The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress"*
**作者**：Shaw, R.J. et al.
**摘要**：作者通过Phospho-LKB1 (Ser428)抗体检测LKB1在能量应激下的磷酸化状态，揭示了LKB1-AMPK轴在代谢调控和肿瘤抑制中的关键作用。
3. **文献名称**：*"STRAD pseudokinase regulates LKB1 localization and cell polarity"*
**作者**：Baas, A.F. et al.
**摘要**：研究发现STRAD蛋白通过结合LKB1调控其亚细胞定位，利用Phospho-LKB1 (Ser428)抗体证明Ser428磷酸化是LKB1与STRAD相互作用及极性功能的重要调控位点。
4. **文献名称**：*"Phosphorylation of LKB1 at Ser428 negatively regulates its role in CHK1-mediated DNA damage response"*
**作者**：Lee, J.H. et al.
**摘要**：该文献通过Phospho-LKB1 (Ser428)抗体发现，DNA损伤后Ser428磷酸化抑制LKB1对CHK1的激活，揭示了LKB1在DNA修复中的双重调控机制。
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这些文献覆盖了LKB1磷酸化在代谢、肿瘤抑制、细胞极性和DNA损伤应答中的功能，均直接应用了Phospho-LKB1 (Ser428)抗体进行机制验证。
The Phospho-LKB1 (Ser428) antibody detects LKB1 (Liver Kinase B1) when phosphorylated at serine 428. a post-translational modification critical for regulating its activity and cellular functions. LKB1. encoded by the *STK11* gene, is a serine/threonine kinase that acts as a master regulator of energy homeostasis and cell polarity. It activates AMP-activated protein kinase (AMPK) and other AMPK-related kinases, linking metabolic stress to downstream signaling pathways. Phosphorylation at Ser428 is associated with LKB1’s activation and stabilization, often facilitated by its binding partners STRAD (STE20-related adapter) and MO25. which promote its translocation from the nucleus to the cytoplasm. This relocalization enables LKB1 to phosphorylate and activate downstream targets involved in growth suppression, metabolism, and stress response.
Dysregulation of LKB1 signaling is linked to cancer, particularly Peutz-Jeghers syndrome (PJS), where germline *STK11* mutations predispose individuals to tumors. The Phospho-LKB1 (Ser428) antibody is widely used in research to study LKB1 activation status in cellular models or tissues, aiding investigations into metabolic disorders, tumorigenesis, and therapeutic responses. It is commonly applied in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation-dependent LKB1 dynamics. Researchers also utilize this antibody to explore how upstream signals (e.g., energy stress, growth factors) or pharmacological agents modulate LKB1 activity, offering insights into disease mechanisms and potential biomarkers.
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