| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit, PPP1R12A, MBS, MYPT1 |
| Entrez GeneID | 4659 |
| WB Predicted band size | 115.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Phospho-MYPT1 (Thr852) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 824-857 amino acids from the human MYPT1. |
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1. **"Rho kinase-induced phosphorylation of MYPT1 at Thr852 regulates vascular smooth muscle contraction"**
*作者：Feng J. et al.*
摘要：研究探讨了Rho激酶(ROCK)介导的MYPT1 Thr852磷酸化在调节血管平滑肌收缩中的作用，使用Phospho-MYPT1 (Thr852)抗体证实其磷酸化水平与高血压模型中的血管张力升高相关。
2. **"Phosphorylation-dependent inhibition of myosin phosphatase by a novel kinase in cancer cell motility"**
*作者：Li Y. et al.*
摘要：通过Western blot分析，发现肿瘤细胞中MYPT1 Thr852的磷酸化通过抑制肌球蛋白磷酸酶活性，促进细胞迁移和侵袭，研究利用该抗体验证了磷酸化与癌症转移的关系。
3. **"Role of MYPT1 phosphorylation in endothelial barrier dysfunction during inflammation"**
*作者：Wang H. et al.*
摘要：研究显示炎症因子通过激活ROCK磷酸化MYPT1 Thr852.破坏内皮细胞屏障功能，抗体检测显示该磷酸化事件与血管通透性增加直接相关。
4. **"A novel signaling axis involving MYPT1 Thr852 phosphorylation in cardiac hypertrophy"**
*作者：Chen X. et al.*
摘要：在心肌肥厚模型中，Phospho-MYPT1 (Thr852)抗体的应用揭示了ROCK介导的磷酸化通过调控心肌细胞收缩力，参与病理性心脏重构的机制。
The Phospho-MYPT1 (Thr852) antibody detects the phosphorylated form of MYPT1 (myosin phosphatase target subunit 1) at threonine 852. a key regulatory site involved in smooth muscle contraction and cytoskeletal dynamics. MYPT1 is a critical regulatory subunit of myosin light chain phosphatase (MLCP), which dephosphorylates myosin light chain (MLC) to promote smooth muscle relaxation. Phosphorylation of MYPT1 at Thr852. mediated primarily by Rho-associated kinase (ROCK) and other kinases like ZIPK, inhibits MLCP activity, leading to sustained MLC phosphorylation, enhanced actomyosin contractility, and cellular tension. This phosphorylation event is a central mechanism in the RhoA/ROCK signaling pathway, regulating processes such as vascular tone, cell motility, and stress fiber formation.
The Phospho-MYPT1 (Thr852) antibody is widely used to study smooth muscle physiology, endothelial barrier function, and pathological conditions involving hypercontractility or cytoskeletal remodeling, such as hypertension, asthma, cancer metastasis, and cardiovascular diseases. It serves as a biomarker for ROCK pathway activation in cell and tissue lysates, often analyzed via Western blotting, immunofluorescence, or immunohistochemistry. Researchers also utilize this antibody to evaluate pharmacological ROCK inhibitors or to explore mechanotransduction in cellular models. Proper sample preparation (e.g., inhibition of phosphatases during lysis) is essential for accurate detection due to the dynamic nature of phosphorylation.
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