| WB | 1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Melanocortin receptor 4, MC4-R, mc4r |
| Entrez GeneID | 286833 |
| WB Predicted band size | 36.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE mc4r antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57 amino acids from the human region of DANRE mc4r. |
+ +
以下是关于斑马鱼(Danio rerio)MC4R(N-Term)抗体的参考文献示例(内容为虚构，仅供格式参考)：
---
1. **"Characterization of MC4R expression in zebrafish brain using a novel N-terminal antibody"**
*Author: Smith J, et al.*
摘要：本研究利用针对斑马鱼MC4R蛋白N端区域的多克隆抗体，通过免疫组化技术定位了MC4R在斑马鱼下丘脑及周边神经核团的表达，揭示了其在能量调节通路中的潜在作用。
2. **"Functional analysis of MC4R signaling in zebrafish feeding behavior"**
*Author: Lee H, et al.*
摘要：通过N-Term抗体阻断MC4R活性，发现斑马鱼摄食行为显著减少，证实MC4R在能量稳态中的保守功能，并建立了斑马鱼为研究代谢疾病的模型。
3. **"Developmental regulation of mc4r in zebrafish embryos"**
*Author: Garcia R, et al.*
摘要：利用特异性抗体追踪MC4R在斑马鱼胚胎发育中的表达模式，发现其在早期神经发育阶段的动态变化，提示该受体可能参与神经系统的形成。
4. **"Antibody validation for zebrafish MC4R: Applications in pharmacological studies"**
*Author: Wang T, et al.*
摘要：验证了MC4R(N-Term)抗体的特异性，并通过Western blot和免疫荧光证明其在筛选MC4R激动剂/拮抗剂中的实用性，为药物开发提供工具。
---
注：以上文献为示例，实际引用需查询具体数据库(如PubMed)。若需真实文献，可提供更详细关键词或研究背景进一步筛选。
The DANRE MC4R (N-Term) antibody is a specialized tool designed for detecting the melanocortin-4 receptor (MC4R) in zebrafish (*Danio rerio*), targeting its N-terminal region. MC4R, a G protein-coupled receptor, plays a pivotal role in regulating energy homeostasis, appetite, and body weight across vertebrates. In zebrafish, MC4R is involved in conserved physiological pathways, making it a valuable model for studying metabolic disorders and obesity-related mechanisms. The N-terminal domain of MC4R is critical for ligand binding and receptor trafficking, and dysfunctions in this region are linked to impaired signaling and metabolic phenotypes.
This antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to localize and quantify MC4R expression in zebrafish tissues, particularly the brain and peripheral organs. Its development supports comparative studies to unravel evolutionary conservation or divergence in MC4R function between teleosts and mammals. Researchers employ this reagent to investigate developmental, pharmacological, or genetic perturbations affecting MC4R pathways, aiding in the discovery of therapeutic targets for metabolic diseases. Validation typically includes testing in MC4R knockout models to confirm specificity, ensuring reliable data for neuroendocrine and metabolic research.
×
