| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nestin, NES |
| Entrez GeneID | 10763 |
| WB Predicted band size | 177.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1389-1416 amino acids from human Nestin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及Nestin (S1409)抗体的参考文献，信息基于公开数据概括：
1. **文献名称**：Nestin expression in embryonic and adult human brain under normal and pathological conditions
**作者**：Dahlstrand J, et al.
**摘要**：研究使用Nestin (S1409)抗体标记人类胚胎及成人大脑中的神经干细胞，发现Nestin在发育早期高表达，成年后局限于特定神经发生区域，并在胶质瘤中重新激活表达。
2. **文献名称**：Identification of a cancer stem cell in human brain tumors
**作者**：Singh SK, et al.
**摘要**：通过Nestin (S1409)抗体结合其他标记物，鉴定脑肿瘤(如胶质母细胞瘤)中的癌症干细胞群体，证实其自我更新和致瘤能力，为靶向治疗提供依据。
3. **文献名称**：Nestin-GFP transgenic mice label skeletal muscle progenitor cells
**作者**：Yablonka-Reuveni Z, et al.
**摘要**：利用Nestin (S1409)抗体验证转基因小鼠模型中骨骼肌祖细胞的Nestin表达，揭示其在肌肉再生中的作用，为组织修复研究提供工具。
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**备注**：以上信息为示例性概括，实际文献可能需通过学术数据库(如PubMed)以"Nestin antibody S1409"为关键词检索具体研究。部分文献可能需关注抗体在方法学中的应用描述(如Western blot/免疫组化验证)。
Nestin (S1409) antibody is a widely used tool in neuroscience and stem cell research, targeting the intermediate filament protein Nestin. Nestin is a Class VI intermediate filament protein expressed predominantly in neural stem/progenitor cells (NSCs/NPCs) during embryonic development. It plays a critical role in maintaining cell structure, regulating intracellular organelle distribution, and modulating cell signaling. Nestin expression is downregulated as neural precursor cells differentiate into mature neurons or glia, making it a key marker for identifying undifferentiated neural stem cells and progenitor cells in the central and peripheral nervous systems.
The S1409 clone is a rabbit polyclonal antibody raised against a recombinant protein corresponding to the C-terminal region of human Nestin. It recognizes endogenous Nestin across multiple species, including human, mouse, and rat, and is validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Its specificity for Nestin helps researchers distinguish neural stem cells from differentiated cell types in tissues, neurosphere cultures, or injury models. Additionally, Nestin is re-expressed in certain pathological conditions, such as brain tumors (e.g., glioblastoma) and regenerative processes, making this antibody valuable in studying neurogenesis, neural repair, and cancer biology. Proper validation with controls (e.g., tissue-specific expression patterns) is essential to confirm antibody specificity in experimental settings.
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