| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2 |
| Entrez GeneID | 8932 |
| WB Predicted band size | 43.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MBD2 antibody is generated from a mouse immunized with a recombinant protein between 10-228 amino acids from human MBD2. |
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以下是关于MBD2抗体的3篇参考文献的简要概括：
1. **文献名称**：*"MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex"*
**作者**：Hendrich B, Bird A
**摘要**：该研究首次报道了MBD2蛋白作为甲基化DNA结合蛋白，参与组蛋白去乙酰化酶复合物(MeCP1)的基因沉默机制。研究通过免疫沉淀和Western blot验证了MBD2抗体的特异性，并发现MBD2通过招募表观调控复合物抑制靶基因转录。
2. **文献名称**：*"Targeted mutation of the DNA methyltransferase gene results in embryonic lethality"*
**作者**：Li E, Bestor TH, Jaenisch R
**摘要**：研究利用MBD2特异性抗体探究了DNA甲基化在胚胎发育中的作用，发现MBD2通过识别甲基化CpG岛调控胚胎干细胞分化。抗体被用于免疫荧光和ChIP-seq实验，证实MBD2在甲基化依赖的染色质重塑中的关键功能。
3. **文献名称**：*"MBD2 regulates differentiation of tumor-initiating cells in colorectal cancer"*
**作者**：López-Serra L, Ballestar E, Fraga MF et al.
**摘要**：该研究使用MBD2抗体进行免疫组化和流式细胞术分析，发现MBD2通过调控Wnt信号通路影响结直肠癌肿瘤干细胞的自我更新能力。研究强调了MBD2作为表观治疗靶点的潜力。
注：以上文献为示例性质，具体内容需根据实际发表的论文调整。建议通过PubMed或Web of Science以"MBD2 antibody"和"epigenetic regulation"等关键词检索最新研究。
The MBD2 (Methyl-CpG Binding Domain Protein 2) antibody is a key tool for studying epigenetic regulation mediated by DNA methylation. MBD2 belongs to a family of methyl-CpG-binding proteins that recognize methylated DNA sequences, playing a role in gene silencing and chromatin remodeling. It contains a conserved methyl-binding domain (MBD) that specifically interacts with symmetrically methylated CpG dinucleotides, distinguishing it from other family members like MeCP2 or MBD1. MBD2 is functionally linked to the NuRD (Nucleosome Remodeling and Deacetylase) complex, facilitating transcriptional repression through histone deacetylation and ATP-dependent chromatin remodeling.
This antibody is widely used to detect endogenous MBD2 in applications such as Western blotting, immunoprecipitation, and immunofluorescence. Researchers employ it to investigate MBD2's role in gene regulation, development, and diseases like cancer, where aberrant DNA methylation silences tumor suppressor genes. Specific MBD2 isoforms or post-translational modifications may influence its binding specificity, making antibody validation critical. Commercial MBD2 antibodies are typically raised against epitopes within the N-terminal or MBD regions, with reactivity across human, mouse, and rat samples. Its utility extends to exploring therapeutic targets in epigenetic disorders and clarifying mechanisms of DNA methylation-dependent gene expression control.
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