| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Glycine N-methyltransferase, 2.1.1.20, GNMT |
| Entrez GeneID | 27232 |
| WB Predicted band size | 32.7kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GNMT antibody is generated from a mouse immunized with a recombinant protein between 1-295 amino acids from the human GNMT. |
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以下是关于GNMT(甘氨酸N-甲基转移酶)抗体的3篇参考文献的简要整理：
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1. **文献名称**：*"Characterization of a monoclonal antibody against human glycine N-methyltransferase and its application in liver disease diagnostics"*
**作者**：Chen YM, et al.
**摘要**：本研究开发了一种针对人源GNMT的单克隆抗体，验证了其特异性及在免疫组化中的应用。通过分析肝癌患者的肝组织样本，发现GNMT表达水平与肿瘤进展呈负相关，提示其可能作为肝癌的潜在生物标志物。
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2. **文献名称**：*"GNMT regulates lipid metabolism through S-adenosylmethionine homeostasis: Insights from antibody-based tissue profiling"*
**作者**：Liao YJ, et al.
**摘要**：利用GNMT特异性抗体进行组织蛋白表达分析，发现GNMT通过调控S-腺苷甲硫氨酸(SAMe)代谢影响肝脏脂质合成。动物实验表明，GNMT缺失会导致脂肪肝，抗体检测为代谢综合征机制研究提供了工具。
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3. **文献名称**：*"Development of a polyclonal antibody for GNMT and its application in detecting epigenetic modifications in hepatitis B-related cirrhosis"*
**作者**：Wang TH, et al.
**摘要**：研究报道了一种高灵敏度的GNMT多克隆抗体制备方法，并用于慢性乙肝肝硬化患者样本检测。结果表明，GNMT表达下降与DNA甲基化异常相关，提示其在肝纤维化进程中的表观遗传调控作用。
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**备注**：以上文献为示例性概括，实际研究需通过学术数据库(如PubMed、Web of Science)检索具体论文。若需真实文献，建议以关键词“GNMT antibody”、“glycine N-methyltransferase immunohistochemistry”进行查询。
The glycine N-methyltransferase (GNMT) antibody is a tool used to detect and study GNMT, a metabolic enzyme primarily expressed in the liver. GNMT plays a critical role in regulating S-adenosylmethionine (SAMe) levels by catalyzing the methylation of glycine using SAMe as a methyl donor. This reaction helps maintain methylation homeostasis, which is vital for processes like detoxification, DNA repair, and epigenetic regulation. Dysregulation of GNMT has been linked to liver diseases (e.g., fibrosis, cancer), diabetes, and hyperhomocysteinemia.
GNMT antibodies, typically developed in rabbits or mice, are designed to target specific epitopes of the human or murine GNMT protein. They are widely used in techniques like Western blotting, immunohistochemistry, and ELISA to quantify GNMT expression in tissues or cell lines. Researchers employ these antibodies to explore GNMT's role in metabolic pathways, its interaction with environmental toxins (e.g., aflatoxin B1), and its potential as a biomarker or therapeutic target. The specificity and sensitivity of GNMT antibodies make them essential for elucidating its dual roles as a tumor suppressor in hepatocarcinogenesis and a modulator of folate metabolism. Recent studies also focus on GNMT's involvement in obesity-related metabolic disorders, highlighting its broader physiological significance.
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