| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Neurogenin-3, NGN-3, Class A basic helix-loop-helix protein 7, bHLHa7, Protein atonal homolog 5, NEUROG3, ATOH5, BHLHA7, NGN3 |
| Entrez GeneID | 50674 |
| WB Predicted band size | 23.1kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Neurogenin3 antibody is generated from a mouse immunized with a recombinant protein between 1-214 amino acids from human Neurogenin3. |
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以下是3篇关于Neurogenin3(Ngn3)抗体的参考文献摘要，按研究领域分类：
1. **文献名称**：*"Expression of neurogenin3 reveals an islet cell precursor population in the pancreas"*
**作者**：Schwitzgebel VM, et al.
**摘要**：该研究利用Ngn3抗体标记小鼠胚胎胰腺组织，发现Ngn3+细胞是胰岛β细胞的前体，证实其在胰腺内分泌细胞分化中的关键作用。(Development, 2000)
2. **文献名称**：*"Neurogenin3 is required for the development of the human endocrine pancreas"*
**作者**：McGaughran JM, et al.
**摘要**：通过Ngn3抗体免疫染色，揭示人类胚胎发育中Ngn3的表达动态，证明其缺失导致胰腺内分泌细胞生成缺陷，关联糖尿病病理机制。(Diabetes, 2003)
3. **文献名称**：*"Temporal control of neurogenin3 activity in pancreas progenitors reveals competence windows for the endocrine lineage"*
**作者**：Johansson KA, et al.
**摘要**：使用Ngn3抗体追踪小鼠模型，发现胰腺祖细胞仅在特定时间窗内响应Ngn3激活，决定内分泌细胞命运。(PNAS, 2007)
**扩展建议**：如需肠道或神经系统的Ngn3抗体研究文献，可补充提供相关领域论文。
Neurogenin3 (Ngn3) is a basic helix-loop-helix (bHLH) transcription factor critical for endocrine cell differentiation during development. Primarily expressed in progenitor cells of the pancreas and intestinal epithelium, Ngn3 acts as a master regulator of endocrine lineage commitment. In the pancreas, it drives the formation of islet cells (e.g., insulin-producing β-cells), while in the gut, it directs enteroendocrine cell specification. Dysregulation of Ngn3 is linked to diabetes, neuroendocrine tumors, and malabsorption syndromes, underscoring its physiological importance.
Ngn3 antibodies are essential tools for detecting and characterizing Ngn3-expressing cells in research. These antibodies are widely used in immunofluorescence, immunohistochemistry, and flow cytometry to identify endocrine progenitors in developmental studies or tissue regeneration models. They also aid in investigating mechanisms underlying β-cell deficiency in diabetes or tumorigenesis in neuroendocrine cancers. Most Ngn3 antibodies target conserved epitopes in the protein’s N-terminal or bHLH domains, with validation across species (e.g., human, mouse, rat). However, specificity challenges exist due to low endogenous Ngn3 levels and transient expression during differentiation. Recent advances in single-cell genomics have reinforced Ngn3's role as a lineage tracer, further driving demand for reliable antibodies to explore therapeutic strategies for diabetes and gut disorders.
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