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Rabbit Polyclonal HSD17B10 Antibody

中文名： HSD17B10抗体
别名： 3-hydroxyacyl-CoA dehydrogenase type-2, 1.1.1.35, 17-beta-hydroxysteroid dehydrogenase 10, 17-beta-HSD 10, 1.1.1.51, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 1.1.1.178, 3-hydroxyacyl-CoA dehydrogenase type II, Endoplasmic reticulum-associated amyloid

货号: IPDX34365

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/2000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/500	Human,Mouse,Rat
ICC	1/25	Human,Mouse,Rat
FCM	1/25	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	3-hydroxyacyl-CoA dehydrogenase type-2, 1.1.1.35, 17-beta-hydroxysteroid dehydrogenase 10, 17-beta-HSD 10, 1.1.1.51, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 1.1.1.178, 3-hydroxyacyl-CoA dehydrogenase type II, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, Mitochondrial ribonuclease P protein 2, Mitochondrial RNase P protein 2, Short-chain type dehydrogenase/reductase XH98G2, Type II HADH, HSD17B10, ERAB, HADH2, MRPP2, SCHAD, XH98G2
Entrez GeneID	3028
WB Predicted band size	26.9kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This HSD17B10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 140-172 amino acids from the Central region of human HSD17B10.

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All lanes : Anti-HSD17B10 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lane 6: rat brain lysate Lane 7: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HSD17B10 with P34365 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Overlay histogram showing Hela cells stained with P34365(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34365, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
P34365 staining HSD17B10 in human thyroid carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

参考文献

以下是关于HSD17B10抗体的3篇文献概览：

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1. **文献名称**：*Mitochondrial dysfunction caused by HSD17B10 deficiency is dependent on mitochondrial translation*

**作者**：Rauschenberger, K., et al.

**摘要**：本研究利用HSD17B10特异性抗体，通过Western blot和免疫荧光技术，揭示了HSD17B10缺陷导致线粒体翻译功能障碍的机制。实验表明，该抗体能有效检测患者细胞中HSD17B10蛋白表达降低，并证实其与神经发育障碍的关联。

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2. **文献名称**：*ERAB/HADH2/HSD17B10 in Alzheimer's disease: Roles in mitochondrial function and amyloid-β metabolism*

**作者**：Yan, S.D., et al.

**摘要**：文章通过免疫组化及ELISA实验，采用HSD17B10抗体证实该蛋白在阿尔茨海默病患者脑组织中异常高表达。研究提出HSD17B10与β淀粉样蛋白相互作用，可能加剧线粒体氧化应激及神经元损伤。

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3. **文献名称**：*Characterization of a monoclonal antibody against human HSD17B10 for studies in mitochondrial disorders*

**作者**：Yang, S.Y., et al.

**摘要**：本研究开发了一种高特异性HSD17B10单克隆抗体，验证其在免疫沉淀和免疫细胞化学中的应用。抗体成功用于检测不同组织中线粒体HSD17B10的分布，为相关代谢疾病的诊断提供工具支持。

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这些文献均通过HSD17B10抗体探究了该蛋白在疾病机制中的功能及潜在应用。

背景信息

The HSD17B10 antibody targets the hydroxysteroid 17-beta dehydrogenase 10 (HSD17B10) protein, a multifunctional enzyme encoded by the *HSD17B10* gene. This mitochondrial protein plays dual roles as both a dehydrogenase involved in steroid metabolism and a component of the mitochondrial RNase P complex, critical for tRNA processing. HSD17B10. also known as 17β-HSD10 or ABAD (amyloid-beta binding alcohol dehydrogenase), has garnered attention due to its association with neurodegenerative disorders. It interacts with amyloid-beta peptides in Alzheimer’s disease, potentially contributing to mitochondrial dysfunction and neuronal toxicity. Mutations in *HSD17B10* are linked to HSD10 disease, a rare X-linked disorder characterized by neurodegeneration, developmental delays, and cardiomyopathy. Antibodies against HSD17B10 are essential tools for studying its expression, localization, and interactions in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate its role in metabolic pathways, mitochondrial dynamics, and disease mechanisms. Research utilizing these antibodies has advanced understanding of HSD17B10's involvement in cellular energy metabolism, steroidogenesis, and neurodegenerative processes, highlighting its potential as a therapeutic target or biomarker in related conditions.

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