Rabbit Polyclonal PGAP1(N-Term) Antibody

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     All lanes : Anti-PGAP1 Antibody (N-Term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lane 4: mouse kidney lysate Lane 5: mouse testis lysate Lane 6: rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PGAP1 with P34362 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing Hela cells stained with P34362(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34362,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight®488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The PGAP1 (N-Term) antibody is a tool used to detect the N-terminal region of the protein PGAP1 (Post-GPI Attachment to Proteins 1), which plays a critical role in the processing and maturation of glycosylphosphatidylinositol (GPI)-anchored proteins. GPI anchors are lipid modifications that tether proteins to cell membranes, and their proper attachment is essential for protein localization and function. PGAP1. an endoplasmic reticulum (ER)-resident enzyme, catalyzes the cleavage of the GPI anchor’s lipid moiety during protein remodeling, a step required for efficient transport of GPI-anchored proteins from the ER to the Golgi apparatus.

Research on PGAP1 has implications for understanding cellular trafficking mechanisms, membrane protein dynamics, and diseases linked to GPI anchor deficiencies, such as certain forms of intellectual disability and metabolic disorders. The PGAP1 (N-Term) antibody is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study PGAP1 expression, subcellular localization, and interactions. By targeting the N-terminal region, this antibody helps distinguish full-length PGAP1 from potential cleavage products or isoforms. Validation of specificity, including knockdown controls or peptide blocking assays, is critical to ensure accurate detection in experimental models. Its application contributes to elucidating PGAP1’s role in cellular physiology and pathology.