Rabbit Polyclonal EEF1A1P5 Antibody

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     All lanes : Anti-EEF1A1P5 Antibody (C-Term) at 1:1000-1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: 293 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EEF1A1P5 with P34361 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG  secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing HepG2 cells stained with P34361(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34361,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed. .    

The EEF1A1P5 antibody targets a protein product associated with the *EEF1A1P5* pseudogene, a non-functional, evolutionarily conserved relative of the *EEF1A1* gene. *EEF1A1* encodes eukaryotic elongation factor 1-alpha 1 (eEF1A1), a critical GTPase involved in protein synthesis by delivering aminoacyl-tRNAs to ribosomes. While *EEF1A1P5* is a processed pseudogene (lacking introns and regulatory regions), it may regulate *EEF1A1* expression through competitive RNA interactions or transcriptional interference.

EEF1A1P5 antibodies are primarily research tools used to study pseudogene-derived proteins or cross-reactive epitopes shared with eEF1A1. These antibodies aid in exploring pseudogene functionality, particularly in cancer, neurodegeneration, and cellular stress, where dysregulated pseudogene expression is increasingly linked to disease mechanisms. For instance, aberrant *EEF1A1P5* activity has been implicated in tumor progression and chemoresistance, possibly via interactions with oncogenic signaling pathways.

However, specificity remains a challenge, as pseudogene-encoded proteins often share high homology with their parental genes. Researchers must validate EEF1A1P5 antibodies rigorously to distinguish pseudogene-specific signals from eEF1A1 cross-reactivity. Despite this, such antibodies provide insights into the regulatory roles of pseudogenes and their potential as biomarkers or therapeutic targets in precision medicine.