Rabbit Polyclonal OVGP1(N-Term) Antibody

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     All lanes : Anti-OVGP1 Antibody (N-Term) at 1:2000 dilution Lane 1: human placenta lysate Lane 2: human uterus lysate Lane 3: human ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing Hela cells stained with P34360(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34360,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P34360 staining OVGP1 in human fallopian tube tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The OVGP1 (N-Term) antibody is a specialized tool used to detect the N-terminal region of Oviductal Glycoprotein 1 (OVGP1), a glycoprotein primarily secreted by the epithelial cells of the mammalian oviduct (fallopian tube in humans). OVGP1. also known as MUC9. plays a critical role in reproductive biology, particularly in fertilization and early embryo development. It facilitates sperm capacitation, protects oocytes, and supports zygote transport by forming a protective microenvironment. The protein’s structure includes conserved mucin-like domains and glycosylation sites, contributing to its functional versatility.

The antibody is typically generated in host species like rabbits or mice using synthetic peptides or recombinant proteins corresponding to the N-terminal region of OVGP1. It is widely utilized in techniques such as Western blotting, immunohistochemistry, and ELISA to study OVGP1 expression patterns in reproductive tissues, aiding research on fertility, embryo implantation, and oviduct-related pathologies. Its specificity for the N-terminal domain ensures targeted detection, minimizing cross-reactivity.

OVGP1 dysregulation has been linked to conditions like ectopic pregnancy, endometriosis, and ovarian cancer, making this antibody valuable in both basic and clinical research. Validations often include checks for reactivity across species (e.g., human, mouse) and tissue specificity to ensure reliability in diverse experimental models.