| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Natural resistance-associated macrophage protein 2, NRAMP 2, Divalent cation transporter 1, Divalent metal transporter 1, DMT-1, Solute carrier family 11 member 2, SLC11A2, DCT1, DMT1, NRAMP2 |
| Entrez GeneID | 4891 |
| WB Predicted band size | 62.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SLC11A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-291 amino acids from the Central region of human SLC11A2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SLC11A2(DMT1)抗体的虚构参考文献示例，格式符合要求：
1. **"Characterization of SLC11A2 in iron transport using a novel polyclonal antibody"**
*Gunshin H., et al. (2005), Journal of Biological Chemistry*
摘要：开发了一种兔源多克隆抗体，通过Western blot和免疫组化验证SLC11A2在小肠上皮细胞和肝脏中的表达，证实其在二价金属离子跨膜转运中的关键作用。
2. **"SLC11A2 mediates macrophage iron recycling: Evidence from antibody-based knockout models"**
*Fleming R.E., Lei P. (2012), Blood*
摘要：利用特异性单克隆抗体阻断SLC11A2功能，揭示巨噬细胞铁再循环缺陷与炎症性贫血的关联，抗体染色显示该蛋白在溶酶体膜特异性定位。
3. **"Differential expression of SLC11A2 isoforms in neuronal cells revealed by epitope-specific antibodies"**
*Shawki A., McKenzie J. (2018), Cell Reports*
摘要：设计针对SLC11A2两种剪接变体(IRE+/IRE-)的抗体，发现神经元中IRE+亚型高表达，提示其在中枢神经系统铁稳态中的特殊调控机制。
注：以上文献为示例性质，实际引用需以真实出版物为准。建议通过PubMed或Google Scholar以"SLC11A2/DMT1 antibody"为关键词检索最新研究。
The SLC11A2 antibody is a tool used to detect and study the solute carrier family 11 member 2 (SLC11A2), also known as the divalent metal transporter 1 (DMT1). This transmembrane protein plays a critical role in cellular iron homeostasis by transporting ferrous iron (Fe²⁺) and other divalent metals (e.g., manganese, copper) across endosomal membranes or the plasma membrane. SLC11A2 is essential for dietary iron absorption in the duodenum, iron recycling in macrophages, and iron uptake in erythroid precursor cells. Dysregulation of SLC11A2 is linked to iron-related disorders, including iron-deficiency anemia, hemochromatosis, and neurodegenerative diseases associated with metal accumulation.
Antibodies targeting SLC11A2 are widely used in research to investigate its expression, localization, and function in tissues and cell lines. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels under varying physiological or pathological conditions. Some antibodies distinguish between SLC11A2 isoforms (e.g., IRE and non-IRE variants) or post-translational modifications. Validation via knockout controls or siRNA knockdown is critical to confirm specificity. These reagents have advanced studies on iron metabolism, metal toxicity, and therapeutic targeting of iron-related diseases. Commercial SLC11A2 antibodies are typically raised in rabbits or mice, with clonal variants offering distinct sensitivity and cross-reactivity profiles.
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