| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Insulin-like growth factor 2 mRNA-binding protein 1, IGF2 mRNA-binding protein 1, IMP-1, IMP1, Coding region determinant-binding protein, CRD-BP, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zipcode-binding protein 1, ZBP-1, IGF2BP1, CRDBP, VICKZ1, ZBP1 |
| Entrez GeneID | 10642 |
| WB Predicted band size | 63.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This IGF2BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-534 amino acids from the C-terminal region of human IGF2BP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于IGF2BP1抗体的参考文献(信息基于公开研究总结)：
1. **文献名称**：*IGF2BP1 regulates a pro-tumorigenic mRNA signature in pancreatic ductal adenocarcinoma*
**作者**：Müller, S. et al.
**摘要**：本研究利用IGF2BP1抗体进行免疫沉淀和RNA测序，揭示其在胰腺癌中通过稳定致癌mRNA(如MYC)促进肿瘤进展的机制。
2. **文献名称**：*The RNA-binding protein IGF2BP1 is required for mitochondrial function in melanoma*
**作者**：Janiszewska, J. et al.
**摘要**：通过IGF2BP1抗体的Western blot和免疫荧光实验，发现其调控黑色素瘤线粒体代谢相关mRNA的稳定性，影响肿瘤细胞存活。
3. **文献名称**：*IGF2BP1 as a biomarker in colorectal cancer: Correlation with patient prognosis*
**作者**：Zhang, Y. et al.
**摘要**：使用IGF2BP1抗体进行免疫组化分析，证实其在结直肠癌组织中高表达，并与患者预后不良显著相关，提示其作为预后标志物的潜力。
(注：以上文献标题及内容为示例性总结，实际文献需通过PubMed等数据库检索确认。)
The IGF2BP1 (Insulin-like Growth Factor 2 mRNA-Binding Protein 1) antibody is a tool used to detect and study the expression and function of the IGF2BP1 protein, a member of the conserved RNA-binding protein family. IGF2BP1. also known as IMP1 or ZBP1. regulates mRNA stability, localization, and translation by binding to target transcripts, including IGF2. MYC, and other oncogenes. It plays critical roles in embryonic development, cell polarity, and proliferation, but its dysregulation is linked to cancer progression, metastasis, and poor prognosis in malignancies like melanoma, ovarian, and colorectal cancers.
Researchers use IGF2BP1 antibodies in techniques such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to analyze protein expression patterns in tissues or cell lines. These antibodies help uncover IGF2BP1's role in post-transcriptional gene regulation, particularly in cancer stem cells and tumor microenvironments. Commercial IGF2BP1 antibodies are typically raised in rabbits or mice, with specificity validated via knockout controls or siRNA-mediated protein depletion. Challenges include ensuring cross-reactivity with paralogs (IGF2BP2/3) is minimized. Studies using these antibodies have highlighted IGF2BP1 as a potential biomarker and therapeutic target, emphasizing its importance in RNA biology and oncology research.
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