| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Suppressor of fused homolog, SUFUH, SUFU |
| Entrez GeneID | 51684 |
| WB Predicted band size | 53.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, African Green Mouseonkey, Mouse |
| Immunogen | This SUFU antibody is generated from a mouse immunized with a recombinant protein of human SUFU. |
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以下是3条关于SUFU抗体的参考文献示例(内容为虚构，仅供示例用途)：
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1. **文献名称**：*SUFU Antibody Validation in Hedgehog Pathway Inhibition Studies*
**作者**：Lee, J. et al.
**摘要**：本研究验证了一种高特异性SUFU抗体在Western blot和免疫荧光中的应用，证明其在Hedgehog信号通路中能有效检测SUFU蛋白与Gli转录因子的相互作用，为肿瘤微环境研究提供可靠工具。
2. **文献名称**：*SUFU as a Prognostic Marker in Medulloblastoma: Antibody-Based Detection*
**作者**：Garcia, R. & Martinez, P.
**摘要**：通过新型SUFU抗体对髓母细胞瘤组织样本进行免疫组化分析，发现SUFU低表达与患者预后不良显著相关，提示其作为临床生物标志物的潜力。
3. **文献名称**：*Development of a Monoclonal Antibody Targeting SUFU for Functional Studies*
**作者**：Chen, X. et al.
**摘要**：报道一种针对SUFU C端结构域的单克隆抗体的开发，验证其在Co-IP和流式细胞术中的特异性，并揭示SUFU在基底细胞癌中的异常降解机制。
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注：以上文献信息为模拟生成，实际引用需查询PubMed、Google Scholar等数据库获取真实文献。如需具体文献推荐，建议补充研究背景或应用场景。
Sufu (Suppressor of Fused) is a key regulatory protein in the Hedgehog (Hh) signaling pathway, which plays a critical role in embryonic development, tissue homeostasis, and cell proliferation. As a negative regulator, Sufu binds to Gli transcription factors (Gli1. Gli2. Gli3) to sequester them in the cytoplasm, preventing their nuclear translocation and subsequent activation of target genes. Dysregulation of Sufu is implicated in various cancers, including medulloblastoma and basal cell carcinoma, as well as developmental disorders.
Sufu antibodies are essential tools for studying the molecular mechanisms of Hh signaling. They enable detection of Sufu expression levels, post-translational modifications (e.g., phosphorylation), and interactions with binding partners like Gli proteins. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and co-immunoprecipitation. Additionally, Sufu antibodies aid in exploring its tumor-suppressive role, as loss-of-function mutations or reduced Sufu expression correlate with pathway hyperactivation.
Most commercial Sufu antibodies are raised against conserved epitopes in humans, mice, or other model organisms, with validation across species and applications. Researchers rely on these reagents to dissect Sufu's dual role—both as a pathway inhibitor and a potential stabilizer of Gli proteins—in context-dependent cellular responses. Their utility extends to preclinical studies targeting Hh-driven malignancies, emphasizing Sufu's therapeutic relevance.
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