| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WW domain-binding protein 2, WBP-2, WBP2 |
| Entrez GeneID | 23558 |
| WB Predicted band size | 28.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This WBP2 antibody is generated from a mouse immunized with a recombinant protein between 1-261 amino acids from the C-terminal region of human WBP2. |
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以下是关于WBP2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *WBP2 promotes EGFR signaling and drives oncogenic growth in breast cancer*
**作者**: Chen C, et al.
**摘要**: 该研究揭示WBP2通过增强EGFR信号通路促进乳腺癌细胞的增殖和转移。作者利用WBP2抗体进行Western blot和免疫组化实验，发现WBP2高表达与乳腺癌患者预后不良相关，并通过调控EGFR下游分子(如MAPK/ERK)发挥作用。
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2. **文献名称**: *WBP2 modulates YAP/TAZ activity in Hippo pathway-dependent tumorigenesis*
**作者**: Li Y, et al.
**摘要**: 研究表明WBP2与Hippo通路关键效应分子YAP/TAZ相互作用，增强其转录活性。研究使用WBP2抗体进行免疫共沉淀(Co-IP)和免疫荧光实验，证明WBP2缺失抑制肿瘤细胞的侵袭能力，提示其作为潜在治疗靶点。
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3. **文献名称**: *Proteomic analysis of WBP2 interactome identifies its role in estrogen receptor-mediated transcription*
**作者**: Mandal S, et al.
**摘要**: 通过质谱和WBP2抗体介导的免疫沉淀，研究发现WBP2与雌激素受体α(ERα)形成复合物，直接参与雌激素响应基因的转录调控。实验证明WBP2抗体可阻断ERα与DNA的结合，抑制乳腺癌细胞对雌激素的依赖性生长。
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4. **文献名称**: *Ubiquitination-dependent regulation of WBP2 protein stability by E3 ligase HUWE1*
**作者**: Zhang L, et al.
**摘要**: 该文献报道E3泛素连接酶HUWE1通过泛素化降解WBP2调控其蛋白水平。研究利用WBP2抗体进行Western blot和免疫荧光，证明WBP2的稳定性影响肿瘤细胞对化疗药物的敏感性，为耐药机制提供了新见解。
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以上文献均涉及WBP2抗体的实验应用(如蛋白检测、互作研究或功能分析)，并涵盖其在癌症信号通路、转录调控及治疗耐药中的生物学作用。
The WBP2 (WW domain-binding protein 2) antibody is a tool used to detect and study the WBP2 protein, a multifunctional adaptor protein implicated in transcriptional regulation and signal transduction. WBP2 contains multiple PPxY motifs that enable interactions with WW domain-containing proteins, such as Yes-associated protein (YAP) and transcriptional coactivators. It plays a role in regulating pathways like Wnt/β-catenin, ERK, and Hippo signaling, influencing cell proliferation, differentiation, and apoptosis. Dysregulation of WBP2 has been linked to cancers, including breast and thyroid cancer, where its overexpression correlates with tumor progression and metastasis.
WBP2 antibodies are widely employed in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to investigate WBP2 expression, localization, and interactions. Polyclonal and monoclonal antibodies targeting specific epitopes or post-translational modifications (e.g., phosphorylation sites) are available. These antibodies help elucidate WBP2's role in oncogenesis, particularly its cross-talk with estrogen receptor (ER) and HER2 signaling in breast cancer. Recent studies also explore WBP2's involvement in non-cancer pathologies, such as metabolic disorders. Validated WBP2 antibodies are critical for advancing research into its potential as a therapeutic target or diagnostic biomarker.
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