Rabbit Polyclonal TAF4 Antibody

+ +

•  

   
     All lanes : Anti-TAF4 Antibody (C-Term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: U-251 MG whole cell lysate Lane 3: A431 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: CCRF-CEM whole cell lysate Lane 6: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 110 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     P34331 staining TAF4 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

•  

   
     P34331 staining TAF4 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

TAF4 (TATA-box binding protein-associated factor 4) is a critical subunit of the TFIID complex, a general transcription factor essential for RNA polymerase II-mediated gene transcription. As part of the TFIID complex, TAF4 interacts with TATA-binding protein (TBP) and other TAFs to facilitate promoter recognition, chromatin remodeling, and recruitment of coactivators. It plays a role in regulating cell cycle progression, differentiation, and development by modulating the transcription of specific genes. TAF4 also forms a subcomplex with TAF12. contributing to the structural integrity of TFIID. Dysregulation of TAF4 has been linked to cancers and developmental disorders, highlighting its biological importance.

TAF4 antibodies are widely used tools in molecular biology to study TFIID assembly, transcription initiation mechanisms, and gene regulation networks. These antibodies enable detection, localization, and quantification of TAF4 in various applications, including Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF). Commercial TAF4 antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers utilize them to explore TAF4's role in cellular processes, its interaction partners, and its tissue-specific expression patterns. Some studies also focus on TAF4 isoforms (e.g., TAF4b), which exhibit distinct expression profiles in reproductive tissues and cancers. Proper antibody validation remains crucial due to potential cross-reactivity with homologous TAF family members.