关键信息
-
基因名
ADAM33
- 应用
-
别名
ADAM33；ALS2CR4；Transmembrane Protein 237
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9BZ11
-
表达区间
30-701aa
-
氨基酸序列
VLQGHIPGQPVTPHWVLDGQPWRTVSLEEPVSKPDMGLVALEAEGQELLLELEKNHRLLAPGYIETHYGPDGQPVVLAPNHTDHCHYQGRVRGFPDSWVVLCTCSGMSGLITLSRNASYYLRPWPPRGSKDFSTHEIFRMEQLLTWKGTCGHRDPGNKAGMTSLPGGPQSRGRREARRTRKYLELYIVADHTLFLTRHRNLNHTKQRLLEVANYVDQLLRTLDIQVALTGLEVWTERDRSRVTQDANATLWAFLQWRRGLWAQRPHDSAQLLTGRAFQGATVGLAPVEGMCRAESSGGVSTDHSELPIGAAATMAHEIGHSLGLSHDPDGCCVEAAAESGGCVMAAATGHPFPRVFSACSRRQLRAFFRKGGGACLSNAPDPGLPVPPALCGNGFVEAGEECDCGPGQECRDLCCFAHNCSLRPGAQCAHGDCCVRCLLKPAGALCRQAMGDCDLPEFCTGTSSHCPPDVYLLDGSPCARGSGYCWDGACPTLEQQCQQLWGPGSHPAPEACFQVVNSAGDAHGNCGQDSEGHFLPCAGRDALCGKLQCQGGKPSLLAPHMVPVDSTVHLDGQEVTCRGALALPSAQLDLLGLGLVEPGTQCGPRMVCQSRRCRKNAFQELQRCLTACHSHGVCNSNHNCHCAPGWAPPFCDKPGFGGSMDSGPVQAENHDT
-
分子量
78.0 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ADAM33, a member of the ADAM (A Disintegrin and Metalloproteinase) family, has garnered significant attention due to its association with asthma and other allergic diseases. Identified as a candidate gene linked to airway hyperresponsiveness and inflammation, ADAM33 is implicated in the processes of tissue remodeling and inflammation in the respiratory system. Research indicates that ADAM33 is highly expressed in bronchial tissue and is involved in the shedding of various cell surface proteins, including cytokines and adhesion molecules, which play crucial roles in immune response and cell signaling. The protein's extracellular metalloproteinase domain suggests a functional role in proteolytic activity, potentially affecting lung function and inflammatory pathways. Genetic polymorphisms in the ADAM33 gene have been associated with an increased risk of developing asthma, highlighting its relevance in genetic predisposition to respiratory diseases. Given these insights, the recombinant expression and functional characterization of ADAM33 protein could provide vital information in understanding its role in asthma pathophysiology. This research may pave the way for novel therapeutic approaches targeting ADAM33, ultimately improving management strategies for individuals with asthma and related respiratory disorders. Through recombinant protein technology, scientists aim to elucidate the mechanistic actions of ADAM33, contributing to the broader understanding of its significance in airway diseases and enhancing biomarker development for disease prediction and progression monitoring.
