| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Anaphase-promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein, ANAPC11 |
| Entrez GeneID | 51529 |
| WB Predicted band size | 9.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ANAPC11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 56-94 amino acids from human ANAPC11. |
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以下是关于ANAPC11抗体的示例参考文献(注：以下内容为虚构示例，建议通过学术数据库检索真实文献)：
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1. **文献名称**：*ANAPC11 is a critical subunit of the anaphase-promoting complex regulating mitotic exit*
**作者**：Zhang Y. et al.
**摘要**：研究通过ANAPC11抗体进行免疫共沉淀和Western blot分析，揭示了ANAPC11在APC/C复合体组装中的核心作用，并证明其缺失会导致细胞周期停滞在中期。
2. **文献名称**：*Dysregulation of ANAPC11 in colorectal cancer correlates with tumor progression*
**作者**：Lee S. et al.
**摘要**：利用ANAPC11抗体进行免疫组化分析，发现结直肠癌组织中ANAPC11蛋白表达显著升高，且与患者预后不良相关，提示其作为潜在生物标志物的可能性。
3. **文献名称**：*Structural insights into APC/C-mediated ubiquitination via ANAPC11 antibody-based assays*
**作者**：Molina H. et al.
**摘要**：通过ANAPC11抗体结合质谱技术，解析了APC/C复合体的泛素化机制，阐明了ANAPC11与其他亚基的相互作用对底物识别的调控。
4. **文献名称**：*ANAPC11 knockdown impairs neuronal differentiation via antibody-based functional screens*
**作者**：Chen R. et al.
**摘要**：研究使用ANAPC11抗体验证siRNA敲低效果，证明ANAPC11缺失会抑制神经元分化，并影响泛素-蛋白酶体系统的关键底物降解。
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建议通过PubMed或Google Scholar搜索关键词“ANAPC11 antibody”或“ANAPC11 + APC/C”获取真实文献。
The ANAPC11 antibody targets the ANAPC11 protein, a crucial subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for cell cycle regulation. The APC/C controls progression through mitosis and the G1 phase by tagging specific proteins, such as securin and cyclin B, with ubiquitin for proteasomal degradation. ANAPC11. along with ANAPC2. forms the catalytic core responsible for recruiting E2 ubiquitin-conjugating enzymes, enabling substrate ubiquitination.
Structurally, ANAPC11 contains a conserved DOC domain that mediates interactions with APC/C subunits and E2 enzymes. Dysregulation of APC/C activity, including ANAPC11 function, is linked to mitotic errors, genomic instability, and diseases like cancer and neurodegenerative disorders. ANAPC11 overexpression or mutations have been observed in certain tumors, suggesting its role in oncogenesis.
ANAPC11 antibodies are widely used in research to study APC/C assembly, substrate recognition, and cell cycle mechanisms. They facilitate techniques like immunoblotting, immunofluorescence, and co-immunoprecipitation to assess protein expression, localization, and interactions. These tools are critical for exploring ANAPC11's involvement in disease pathways and its potential as a therapeutic target. Commercial ANAPC11 antibodies are typically validated for specificity in human, mouse, or rat models, supporting diverse experimental applications.
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