| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Multivesicular body subunit 12A, CIN85/CD2AP family-binding protein, ESCRT-I complex subunit MVB12A, Protein FAM125A, MVB12A, CFBP, FAM125A |
| Entrez GeneID | 93343 |
| WB Predicted band size | 28.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FAM125A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 152-186 amino acids from the Central region of human FAM125A. |
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以下是关于FAM125A抗体的假设性参考文献示例(请注意，这些文献为虚构，仅作参考用途)：
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1. **文献名称**：**"FAM125A Antibody Validation in Human Tissues: A Tool for Subcellular Localization Studies"**
**作者**：Chen L., et al.
**摘要**：本研究开发并验证了一种兔源多克隆FAM125A抗体，通过免疫组化和Western blot技术证实其在多种人类组织中的特异性。结果显示FAM125A主要定位于细胞核，并与核基质蛋白共定位，提示其在核结构维持中的潜在作用。
2. **文献名称**：**"FAM125A Expression Correlates with Prostate Cancer Progression"**
**作者**：Martinez R., et al.
**摘要**：利用FAM125A抗体对前列腺癌组织微阵列分析发现，FAM125A蛋白表达水平与肿瘤分期及转移显著相关，提示其可能作为癌症预后标志物。机制研究表明FAM125A通过调控AR信号通路促进癌细胞增殖。
3. **文献名称**：**"Functional Analysis of FAM125A in Neuronal Development Using CRISPR/Cas9 and Antibody-Based Approaches"**
**作者**：Tanaka H., et al.
**摘要**：结合CRISPR敲除技术和FAM125A抗体功能阻断实验，揭示FAM125A在神经元轴突导向中的关键作用。免疫荧光显示其与微管结合蛋白相互作用，影响细胞骨架动力学。
4. **文献名称**：**"Cross-Reactivity Evaluation of Commercial FAM125A Antibodies: Implications for Research Reproducibility"**
**作者**：Garcia S., et al.
**摘要**：系统性评估市售5种FAM125A抗体的特异性，发现仅两种通过敲除验证。研究强调抗体选择对数据可靠性的影响，并提供优化实验条件建议(如抗原修复方法)。
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**备注**：实际研究中，建议通过PubMed或Google Scholar以“FAM125A antibody”或“FAM125A protein function”为关键词检索最新文献，并优先选择经同行评审、且抗体验证数据完整的文章。部分研究可能使用FAM125A别名(如KIAA1949)进行发表。
**Background of FAM125A Antibody**
FAM125A, also known as RITA (RBP-J-interacting and tubulin-associated protein) or C10orf104. is a protein encoded by the FAM125A gene located on chromosome 10. It is implicated in cellular processes such as transcriptional regulation, cell cycle control, and microtubule dynamics. Structurally, FAM125A contains conserved nuclear localization signals (NLS) and coiled-coil domains, suggesting roles in nuclear-cytoplasmic shuttling and protein interactions.
The FAM125A antibody is a crucial tool for detecting and studying this protein in research. It has been utilized to investigate FAM125A's interaction with RBP-J (a transcription factor in the Notch signaling pathway) and its association with tubulin, linking it to mitotic regulation. Studies highlight FAM125A's potential tumor-suppressive functions, as its downregulation correlates with poor prognosis in cancers like breast carcinoma and glioblastoma. Additionally, FAM125A may influence neuronal development and neurodegenerative disorders, given its expression in brain tissues.
Antibodies targeting FAM125A (e.g., polyclonal or monoclonal) enable applications such as Western blotting, immunofluorescence, and immunohistochemistry, aiding in protein localization, expression profiling, and mechanistic studies. Validation includes specificity checks via knockdown/knockout controls. Ongoing research aims to clarify FAM125A's precise molecular roles and therapeutic relevance, emphasizing the antibody's utility in advancing cellular and disease-related insights.
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