| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1, PRDM16, KIAA1675, MEL1, PFM13 |
| Entrez GeneID | 63976 |
| WB Predicted band size | 140.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PRDM16 antibody is generated from a mouse immunized with a recombinant protein between 779-996 amino acids from PRDM16. |
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以下是关于PRDM16抗体的3篇参考文献(简略版)：
1. **文献名称**：*PRDM16 Controls a Brown Fat/Skeletal Muscle Switch*
**作者**：Seale P, et al.
**摘要**：该研究通过免疫沉淀和Western blot分析，使用PRDM16抗体证实其在白色脂肪细胞向棕色脂肪细胞分化中的关键作用，揭示了PRDM16与转录复合物的相互作用。
2. **文献名称**：*PRDM16 is Required for the Maintenance of Leukemia Stem Cells in Acute Myeloid Leukemia*
**作者**：Shimizu T, et al.
**摘要**：利用PRDM16抗体进行免疫组化和流式细胞术，研究发现PRDM16在AML白血病干细胞中的高表达，并证明其通过调控下游信号通路维持肿瘤干细胞特性。
3. **文献名称**：*Adipose-Specific Deletion of PRDM16 Results in Lipodystrophy and Insulin Resistance*
**作者**：Cohen S, et al.
**摘要**：通过PRDM16抗体的免疫荧光染色和Western blot，揭示脂肪组织中PRDM16缺失导致线粒体功能受损、脂代谢异常及胰岛素抵抗的分子机制。
(注：若需具体文献来源或DOI，建议在PubMed或期刊官网检索标题或作者名获取全文。)
The PRDM16 (PR/SET Domain 16) antibody is a critical tool for studying the roles of the PRDM16 protein, a transcriptional regulator involved in cell fate determination, metabolism, and oncogenesis. PRDM16 belongs to the PRDI-BF1-RIZ homology domain family, characterized by zinc finger motifs and a catalytic SET domain, though its methyltransferase activity remains debated. It is best known for promoting brown/beige adipocyte differentiation and suppressing white adipocyte genes, making it a focus in obesity and metabolic disease research. In hematopoiesis, PRDM16 acts as an oncogenic driver in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), often through chromosomal translocations.
PRDM16 antibodies are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect protein expression, subcellular localization, and DNA-binding activity. Due to alternative splicing, PRDM16 has multiple isoforms (e.g., full-length and short isoforms lacking the PR/SET domain), necessitating antibodies validated for specific variants. Cross-reactivity with related proteins (e.g., PRDM3/MEL1) is a common concern, emphasizing the need for rigorous validation using knockout controls or siRNA.
Commercial PRDM16 antibodies are typically raised against epitopes in the N-terminal or C-terminal regions, with applications spanning developmental biology, cancer research, and metabolic studies. Understanding its context-dependent roles—as both a tumor suppressor and oncogene—highlights the antibody’s importance in mechanistic and diagnostic investigations.
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