Mouse Monoclonal TPX2 Antibody

The TPX2 (Targeting Protein for Xklp2) antibody is a crucial tool in studying the role of TPX2. a microtubule-associated protein essential for mitotic spindle assembly and cell division. Discovered in the late 1990s, TPX2 is conserved across eukaryotes and functions by activating Aurora A kinase, a key regulator of mitosis. It localizes to spindle microtubules during mitosis, facilitating chromosome segregation and ensuring genomic stability. TPX2’s N-terminal domain binds importins, regulating its nuclear-cytoplasmic shuttling, while its C-terminal domain interacts with microtubules and Aurora A. Dysregulation of TPX2 is linked to cancer, as overexpression promotes uncontrolled proliferation, chromosomal instability, and poor prognosis in various malignancies. TPX2 antibodies (polyclonal or monoclonal) are widely used in techniques like immunofluorescence, Western blotting, and immunohistochemistry to visualize TPX2 expression, localization, and dynamics in cells or tissues. These antibodies aid in elucidating TPX2’s mechanistic roles in mitosis, cancer biology, and potential therapeutic targeting. Validation for specificity (e.g., knockout controls) is critical due to cross-reactivity risks with homologous proteins. Research using TPX2 antibodies continues to advance understanding of cell cycle regulation and oncogenesis.