| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Dual specificity protein phosphatase 22-A, dusp22a {ECO:0000312|ZFIN:ZDB-GENE-040718-219} |
| Entrez GeneID | 436787 |
| WB Predicted band size | 23.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE dusp22a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-195 amino acids from the C-terminal region of DANRE dusp22a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Characterization of dual-specificity phosphatase 22a in zebrafish embryogenesis"** by Smith J. et al.
*摘要*：研究利用抗DUSP22a抗体分析了斑马鱼胚胎中该磷酸酶的时空表达模式，发现其在神经管形成和体节发育阶段高表达，提示其参与MAPK信号通路的调控。
2. **"Antibody validation for zebrafish Dusp22a in oxidative stress response studies"** by Lee H. & Kim S.
*摘要*：报道了针对斑马鱼Dusp22a的多克隆抗体的制备及验证，通过免疫印迹和免疫荧光证实其在肝脏组织中的特异性定位，并发现其表达受氧化应激诱导上调。
3. **"Dusp22a modulates T-cell development in zebrafish via JNK pathway inhibition"** by Garcia R. et al.
*摘要*：利用DANRE Dusp22a抗体研究斑马鱼胸腺中T细胞分化过程，证明Dusp22a通过抑制JNK磷酸化调控免疫细胞发育，抗体染色显示其在淋巴组织中的动态表达。
4. **"Functional divergence of Dusp22 paralogs in teleost development"** by Watanabe T. et al.
*摘要*：比较斑马鱼dusp22a与dusp22b的功能差异，使用特异性抗体揭示dusp22a在早期胚胎的背腹轴形成中起关键作用，而dusp22b更多参与成鱼代谢调控。
注：以上文献为示例，实际引用需根据具体研究检索(如PubMed、Google Scholar)。若研究较少，可扩展至脊椎动物DUSP22相关抗体应用文献。
The DANRE dusp22a antibody is a specialized tool designed to detect and study the dual-specificity phosphatase 22a (dusp22a) protein in the model organism *Danio rerio* (zebrafish). Dusp22a belongs to the dual-specificity phosphatase family, which regulates key signaling pathways, including MAPK (mitogen-activated protein kinase) cascades, by dephosphorylating both tyrosine and serine/threonine residues. This enzyme plays a role in cellular processes such as development, stress responses, and immune regulation. Zebrafish dusp22a is particularly studied for its involvement in embryonic development and organogenesis due to the organism's transparency and genetic tractability.
The antibody is typically generated using immunogenic peptides or recombinant proteins derived from zebrafish dusp22a sequences. It enables researchers to investigate protein expression, localization, and function through techniques like Western blotting, immunohistochemistry, or immunofluorescence. Validation often includes testing in knockout models or knockdown experiments to confirm specificity.
Dusp22a research contributes to understanding conserved signaling mechanisms across vertebrates and may provide insights into human diseases linked to phosphatase dysregulation. The DANRE dusp22a antibody thus serves as a critical reagent in developmental biology and molecular studies, bridging zebrafish models with broader biomedical applications.
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