| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Conserved oligomeric Golgi complex subunit 2, COG complex subunit 2, Component of oligomeric Golgi complex 2, Low density lipoprotein receptor defect C-complementing protein, COG2, LDLC |
| Entrez GeneID | 22796 |
| WB Predicted band size | 83.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This COG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-124 amino acids from the N-terminal region of human COG2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于COG2(N-term)抗体的3篇假设性参考文献及其摘要概括：
1. **"Characterization of a novel COG2 N-terminal antibody reveals its role in Golgi integrity maintenance"**
- **作者**: Smith A, et al.
- **摘要**: 该研究报道了一种针对COG2蛋白N端结构域的多克隆抗体的开发与验证。通过免疫印迹和免疫荧光实验，证实该抗体可特异性识别内源性COG2.并揭示COG2缺失导致高尔基体结构碎片化，提示其在维持高尔基体形态中的关键作用。
2. **"COG complex subunit 2 (COG2) interacts with Syntaxin 5 via its N-terminal domain to regulate vesicular trafficking"**
- **作者**: Miller B, et al.
- **摘要**: 利用COG2(N-term)抗体进行免疫共沉淀实验，发现COG2通过N端与Syntaxin 5直接互作，调控囊泡运输过程。研究进一步通过CRISPR-Cas9敲除COG2.结合抗体标记证实其功能缺失会导致分泌蛋白运输障碍。
3. **"Localization and functional analysis of COG2 in mitotic cells using a domain-specific antibody"**
- **作者**: Zhang Y, et al.
- **摘要**: 本研究通过COG2 N端特异性抗体，发现COG2在有丝分裂期细胞中与高尔基体衍生囊泡共定位，并参与纺锤体组装。抗体阻断实验表明COG2的N端结构域对细胞周期进程具有调控作用。
4. **"Dysregulation of COG2 in neurodegenerative diseases: Insights from antibody-based profiling"**
- **作者**: Johnson R, et al.
- **摘要**: 通过COG2(N-term)抗体对阿尔茨海默病模型脑组织进行分析，发现COG2表达水平显著降低，且与β-淀粉样蛋白沉积相关，提示其在神经退行性疾病中的潜在病理机制。
注：以上文献为示例性内容，实际引用需查询真实数据库(如PubMed)并核实原文信息。
The COG2 (N-term) antibody is a specific immunological tool designed to detect the N-terminal region of the COG2 protein, a critical subunit of the Conserved Oligomeric Golgi (COG) complex. This multi-subunit complex (comprising COG1–COG8) is essential for maintaining Golgi apparatus structure and function, particularly in vesicle-mediated trafficking, glycosylation, and cargo sorting. COG2. along with COG1. forms the lobe A subcomplex, which interacts with other COG subunits and regulatory proteins to ensure proper tethering of transport vesicles to Golgi membranes. Dysregulation of COG2 disrupts Golgi homeostasis, leading to impaired protein trafficking and glycosylation defects linked to congenital disorders of glycosylation (CDGs) and other diseases.
The COG2 (N-term) antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study COG2 expression, localization, and interactions in various biological contexts. Its specificity for the N-terminal epitope ensures minimal cross-reactivity, making it valuable for investigating COG2’s role in Golgi dynamics, cellular trafficking pathways, and disease mechanisms. This antibody has been instrumental in advancing research on Golgi-related pathologies and membrane trafficking networks.
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