| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dihydrofolate reductase, Dhfr |
| Entrez GeneID | 13361 |
| WB Predicted band size | 21.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse DHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the C-terminal region of mouse DHFR. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于小鼠DHFR抗体的模拟参考文献示例(请注意，这些为示例，实际文献需通过学术数据库查询确认)：
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1. **文献名称**: "Characterization of Mouse Dihydrofolate Reductase Expression Using Monoclonal Antibodies"
**作者**: Smith A, et al.
**摘要**: 本研究开发了针对小鼠DHFR的单克隆抗体，验证了其在Western blot和免疫组化中的特异性。结果显示DHFR在小鼠肝脏和肾脏中高表达，为代谢研究提供工具。
2. **文献名称**: "DHFR Inhibition in Murine Cancer Models: Role of Antibody-Based Detection"
**作者**: Jones B, et al.
**摘要**: 通过抗小鼠DHFR抗体分析甲氨蝶呤处理后肿瘤组织中DHFR蛋白水平变化，表明抑制剂通过降低酶稳定性发挥作用，为抗癌机制提供依据。
3. **文献名称**: "Tissue-Specific Localization of DHFR in Mouse Embryos via Immunohistochemistry"
**作者**: Chen L, et al.
**摘要**: 使用特异性抗体研究小鼠胚胎发育中DHFR的分布，发现其在神经管和心脏中高度活跃，提示其在器官形成中的关键作用。
4. **文献名称**: "Validation of a Novel Polyclonal Antibody for Murine DHFR in Flow Cytometry"
**作者**: Kim S, et al.
**摘要**: 报道一种新型多克隆抗体的开发及验证，成功用于流式细胞术检测小鼠T细胞中DHFR表达，助力免疫代谢研究。
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建议通过PubMed或Google Scholar搜索关键词“mouse DHFR antibody”、“murine dihydrofolate reductase immunohistochemistry”等获取真实文献。
The mouse dihydrofolate reductase (DHFR) antibody is a widely used tool in molecular and cellular biology research for detecting and studying the DHFR enzyme in mouse-derived samples. DHFR is a critical enzyme in folate metabolism, catalyzing the reduction of dihydrofolate to tetrahydrofolate, a cofactor essential for nucleotide synthesis and cell proliferation. Its role in DNA/RNA precursor synthesis makes it a target for anticancer and antimicrobial drugs, such as methotrexate.
Mouse DHFR antibodies are typically developed using purified recombinant or native mouse DHFR protein as immunogens, yielding polyclonal or monoclonal antibodies with high specificity. These antibodies enable researchers to investigate DHFR expression, localization, and regulation in mouse models through techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. They are particularly valuable in studies involving DHFR-targeted therapies, gene knockout/overexpression models, or folate metabolism disorders.
Validated antibodies show minimal cross-reactivity with DHFR from other species, ensuring reliability in mouse-specific experiments. Applications extend to cancer research (studying drug resistance mechanisms), developmental biology, and metabolic studies. Many commercial antibodies are available in unconjugated forms or conjugated to markers like HRP or fluorescent tags for multiplex assays. Proper validation using DHFR-deficient cell lines or tissues is recommended to confirm antibody specificity.
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