| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein Wnt-4, WNT4 |
| Entrez GeneID | 54361 |
| WB Predicted band size | 39.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human WNT4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于WNT4抗体的3篇代表性文献示例(部分为虚构内容，仅作格式参考)：
1. **文献名称**: "WNT4 signaling in female reproductive system development: Antibody-based localization analysis"
**作者**: Parr BA, et al.
**摘要**: 通过WNT4特异性抗体进行免疫组织化学分析，揭示WNT4蛋白在小鼠卵巢发育和雌性生殖道分化中的空间表达模式，证实其在性别决定中的关键作用。
2. **文献名称**: "WNT4 overexpression in ovarian cancer and its detection using monoclonal antibodies"
**作者**: Boyer A, et al.
**摘要**: 开发抗人WNT4单克隆抗体，通过Western blot和免疫荧光验证其在卵巢癌细胞系中的高特异性，并发现WNT4过表达与肿瘤侵袭性正相关。
3. **文献名称**: "A novel WNT4 antibody-based assay for early diagnosis of breast cancer subtypes"
**作者**: Saitoh T, Miyoshi K
**摘要**: 研究团队开发了高灵敏度WNT4抗体检测试剂盒，证明其在三阴性乳腺癌患者组织样本中具有诊断价值，且与患者预后显著相关。
注：以上文献信息为示例性质，实际研究需通过PubMed/Google Scholar等平台检索最新文献。建议结合具体研究场景(如疾病模型、实验方法)筛选针对性参考文献。
The WNT4 antibody is a crucial tool for studying the WNT4 protein, a member of the Wnt family of secreted signaling molecules involved in embryonic development, tissue homeostasis, and disease. WNT4 plays a pivotal role in sex determination, kidney development, and female reproductive system formation. It activates both canonical (β-catenin-dependent) and non-canonical Wnt signaling pathways, regulating cell proliferation, differentiation, and organogenesis. Dysregulation of WNT4 is linked to disorders such as Müllerian aplasia, polycystic kidney disease, and certain cancers.
WNT4 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies enable detection of WNT4 expression in tissues (via immunohistochemistry) or cell lysates (via Western blot), aiding research into developmental biology and disease mechanisms. Specific applications include investigating WNT4's role in ovarian follicle development, adrenal gland function, and tumor microenvironment regulation. Validation often involves knockout cell lines to confirm specificity. High-quality WNT4 antibodies are essential for distinguishing between different Wnt isoforms and elucidating context-dependent signaling outcomes, making them valuable in both basic research and potential therapeutic targeting studies.
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