| WB | 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitogen-activated protein kinase 11, MAP kinase 11, MAPK 11, 2.7.11.24, Mitogen-activated protein kinase p38 beta, MAP kinase p38 beta, p38b, Stress-activated protein kinase 2b, SAPK2b, p38-2, MAPK11, PRKM11, SAPK2, SAPK2B |
| Entrez GeneID | 5600 |
| WB Predicted band size | 41.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 155-186 amino acids from human. |
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以下是3篇涉及Phospho-p38 MAPK (Y182)抗体的文献摘要：
1. **"p38 MAPK-mediated phosphorylation of Y182 facilitates oxidative stress-induced cell death"**
*作者：Lee et al., 2015*
摘要：研究揭示p38 MAPK在氧化应激中通过Y182位点的磷酸化激活下游促凋亡通路，抗体用于Western blot验证神经元细胞中的磷酸化水平。
2. **"Targeting phospho-p38 (Y182) in inflammatory arthritis: a biomarker analysis"**
*作者：Smith et al., 2018*
摘要：通过免疫组化检测类风湿关节炎患者滑膜组织中Phospho-p38 (Y182)的表达，发现其与疾病活动度正相关，抗体特异性经肽段竞争实验验证。
3. **"Role of p38 MAPK tyrosine phosphorylation in cardiac hypertrophy"**
*作者：Zhang et al., 2020*
摘要：利用Phospho-Y182抗体发现压力超负荷模型中p38的酪氨酸磷酸化增强，促进心肌肥厚，抗体应用于心肌组织裂解液的磷酸化检测。
4. **"Validation of phospho-specific p38 MAPK antibodies for flow cytometry applications"**
*作者：Johnson & White, 2019*
摘要：系统评估多种p38磷酸化抗体(包括Y182位点)，确认其在外周血单核细胞中检测TNF-α刺激后p38激活的可靠性，强调抗体优化条件。
*注：以上文献信息为模拟示例，实际引用需核对真实出版物。*
The p38 mitogen-activated protein kinase (MAPK) pathway is a critical signaling cascade involved in cellular responses to stress, inflammation, and apoptosis. Activation of p38 MAPK occurs through dual phosphorylation at conserved threonine and tyrosine residues within its activation loop (Thr180 and Tyr182 in humans). Phosphorylation at Tyr182 (Y182) is a key marker of p38 MAPK activation, typically triggered by upstream kinases like MKK3/6 in response to stimuli such as cytokines, osmotic stress, or UV radiation.
The Phospho-p38 MAPK (Y182) antibody is specifically designed to detect this phosphorylation event, enabling researchers to study p38 activation dynamics in various biological contexts. This antibody is widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess pathway activity in cells or tissues. Its applications span research areas including inflammation, cancer, neurodegenerative diseases, and immune responses, where dysregulated p38 signaling is often implicated.
Validation of this antibody typically involves testing in knockout models or phosphatase-treated samples to confirm specificity. Cross-reactivity with related phosphorylated MAPKs (e.g., JNK or ERK) is generally minimal due to sequence specificity. By detecting Y182 phosphorylation, this tool helps elucidate mechanisms underlying cellular stress responses and therapeutic targeting of the p38 pathway in disease.
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