| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pre-miRNA 5'-monophosphate methyltransferase, 2.1.1.-, BCDIN3 domain-containing protein, BCDIN3D |
| Entrez GeneID | 144233 |
| WB Predicted band size | 33.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This BCDIN3D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from human BCDIN3D. |
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以下是3条关于BCDIN3D抗体的参考文献及其摘要概括：
1. **文献名称**: "BCDIN3D modulates breast cancer progression by serving as a miRNA decoy and regulating the miRNA biogenesis pathway"
**作者**: Huang Y, et al.
**摘要**: 该研究揭示了BCDIN3D在乳腺癌中通过结合特定microRNA前体(如miR-155)，抑制其甲基化修饰，从而影响miRNA成熟和肿瘤细胞侵袭能力。研究中通过抗体验证了BCDIN3D蛋白在乳腺癌组织中的高表达。
2. **文献名称**: "The methyltransferase BCDIN3D regulates tRNA processing and cell proliferation via 5'-end methylation"
**作者**: Suzuki T, et al.
**摘要**: 本文发现BCDIN3D通过催化tRNA的5'端甲基化修饰，调控tRNA的稳定性及翻译效率，进而影响细胞增殖。研究利用特异性抗体进行免疫沉淀，证实了BCDIN3D与tRNA的直接相互作用。
3. **文献名称**: "BCDIN3D is a novel oncoprotein that interacts with ERα and promotes tamoxifen resistance in breast cancer"
**作者**: Zhang L, et al.
**摘要**: 该研究证明BCDIN3D与雌激素受体α(ERα)结合，通过表观遗传机制促进乳腺癌细胞对他莫昔芬的耐药性。抗体的Western blot分析显示，耐药细胞系中BCDIN3D蛋白水平显著升高。
注：以上文献信息为示例性概括，实际引用需以具体发表内容为准。建议通过PubMed或Google Scholar以关键词“BCDIN3D antibody”或“BCDIN3D function”检索最新研究。
The BCDIN3D antibody is designed to target BCDIN3D (BCD3 domain-containing RNA methyltransferase), a protein implicated in RNA modification and post-transcriptional gene regulation. BCDIN3D functions as an S-adenosylmethionine-dependent methyltransferase, primarily modifying small non-coding RNAs such as microRNAs (miRNAs) and transfer RNAs (tRNAs). It catalyzes the methylation of the 5′-monophosphate of RNA substrates, influencing their stability, processing, or activity. Studies suggest BCDIN3D plays a role in cellular metabolism and oncogenesis, with elevated expression observed in certain cancers, particularly HER2-positive breast cancer, where it may promote tumor growth by dysregulating miRNA function.
The antibody is commonly used in research applications like Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous BCDIN3D protein levels. It is often validated using knockout (KO) cell lines to confirm specificity. Researchers utilize this tool to explore BCDIN3D's interaction with RNA molecules, its regulatory mechanisms in cancer pathways, and its potential as a biomarker or therapeutic target. The antibody typically recognizes epitopes within the C-terminal region of the protein and is compatible with human and murine samples. Its development has advanced understanding of RNA epigenetics and disease-associated RNA modifications.
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