Rabbit Polyclonal PRPF6(N-term) Antibody

The PRPF6 (N-term) antibody is a tool used to detect the N-terminal region of Pre-mRNA Processing Factor 6 (PRPF6), a conserved component of the spliceosome involved in pre-mRNA splicing. PRPF6 is essential for assembling and stabilizing the catalytic core of the spliceosome, particularly during the transition from the pre-catalytic B complex to the activated Bact complex. It interacts with other splicing factors, including PRPF8 and SNRNP200. to mediate RNA-RNA and RNA-protein interactions critical for splice site recognition and intron removal. The antibody is typically raised in rabbits or mice using synthetic peptides or recombinant proteins corresponding to the N-terminal domain. It is widely applied in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study PRPF6 expression, localization, and molecular interactions. Researchers use it to explore PRPF6's roles in RNA processing, gene regulation, and its potential links to diseases such as cancer or neurodegenerative disorders. Specificity for the N-terminal region helps avoid cross-reactivity with other spliceosomal proteins, though validation via knockout/knockdown controls is recommended. Its utility extends to investigating splicing dysregulation mechanisms and therapeutic targeting of spliceosome-related pathologies.