| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Spermatogenesis-associated protein 21, SPATA21 |
| Entrez GeneID | 374955 |
| WB Predicted band size | 52.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SPATA21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-424 amino acids from the C-terminal region of human SPATA21. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SPATA21抗体的3篇文献示例(部分内容为模拟，实际文献需根据数据库检索结果补充)：
1. **文献名称**：*SPATA21 is essential for spermatogenesis and male fertility in mice*
**作者**：Li W, et al.
**摘要**：本研究通过构建SPATA21基因敲除小鼠模型，发现其缺失导致精子畸形和雄性不育。利用SPATA21抗体进行免疫荧光和Western blot分析，证实该蛋白在精子顶体形成中的关键作用，并揭示其与微管组织相关的分子机制。
2. **文献名称**：*Characterization of SPATA21 expression in human testicular tissues*
**作者**：Zhang Y, et al.
**摘要**：通过SPATA21抗体对正常和少精症患者的睾丸组织进行免疫组化分析，发现SPATA21在精子细胞阶段特异性高表达，且其表达水平与精子数量呈正相关，提示其可作为男性不育的生物标志物。
3. **文献名称**：*Proteomic identification of SPATA21-interacting proteins during spermiogenesis*
**作者**：Chen H, et al.
**摘要**：采用SPATA21抗体进行免疫共沉淀结合质谱分析，鉴定出SPATA21与多个精子形成相关蛋白(如TCTE1、IQCG)存在相互作用，为阐明其在精子尾部组装中的功能网络提供了实验证据。
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**备注**：以上文献为示例模板，实际引用时需通过PubMed、Web of Science等数据库检索真实文献。若需具体文献支持，建议使用关键词“SPATA21 antibody”或“SPATA21 function”进行精确检索。
The SPATA21 (spermatogenesis-associated protein 21) antibody is a research tool used to study the SPATA21 protein, encoded by the SPATA21 gene located on human chromosome 1q24.2. SPATA21 is predominantly expressed in the testis and plays a critical role in spermatogenesis, particularly during spermatid development and sperm maturation. It is implicated in the structural organization of the sperm head and tail, as well as acrosome formation. Dysregulation of SPATA21 has been associated with male infertility due to its impact on sperm motility and morphology.
SPATA21 antibodies are typically developed in rabbits or mice using immunogenic peptides or recombinant protein fragments specific to the target epitope. These antibodies enable the detection and localization of SPATA21 in tissues or cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Validation includes testing for specificity using knockout controls or siRNA-mediated knockdown.
Research utilizing SPATA21 antibodies has enhanced understanding of molecular mechanisms underlying sperm development and infertility disorders. However, commercial availability remains limited, and most applications are confined to academic studies. Further investigations are needed to explore its interactions with other spermatogenesis-related proteins and potential clinical diagnostic or therapeutic implications.
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