| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Factor in the germline alpha, FIGalpha, Class C basic helix-loop-helix protein 8, bHLHc8, Folliculogenesis-specific basic helix-loop-helix protein, Transcription factor FIGa, FIGLA, BHLHC8 |
| Entrez GeneID | 344018 |
| WB Predicted band size | 24.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FIGLA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-137 amino acids from the Central region of human FIGLA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FIGLA抗体的参考文献及其摘要：
- **"FIGLA, a basic helix-loop-helix transcription factor, balances differentiation and survival of mouse oocytes during postnatal development"**
*作者：Soyal SM, Amleh A, Dean J*
摘要：该研究利用FIGLA抗体检测小鼠卵巢中FIGLA蛋白的表达模式，揭示其在调控卵母细胞分化和存活中的双重作用，基因敲除导致原始卵泡形成缺陷。
- **"FIGLA regulates primordial follicle formation by promoting transcription of oocyte-specific genes"**
*作者：Zhang Y, Zhang L, Sun XX, et al.*
摘要：通过Western blot和免疫荧光技术结合FIGLA抗体，研究证明FIGLA通过激活卵母细胞特异性基因(如Nobox和Gdf9)驱动原始卵泡形成，并影响卵巢储备功能。
- **"Dynamic expression of FIGLA during early ovarian development and its interaction with SOHLH1"**
*作者：Wang H, Liu K, Ji Y, et al.*
摘要：研究使用FIGLA抗体进行免疫组化和共聚焦显微镜分析，发现FIGLA与SOHLH1协同调控卵母细胞发育关键通路，尤其在减数分裂启动阶段起核心作用。
- **"FIGLA modulates granulosa cell apoptosis and autophagy via PI3K/AKT/mTOR signaling"**
*作者：Li J, Chen Q, Zhang R, et al.*
摘要：通过免疫组化和流式细胞术结合FIGLA抗体，该文献揭示FIGLA缺失导致颗粒细胞过度凋亡，其机制可能与PI3K/AKT/mTOR信号通路异常激活相关。
以上研究均通过FIGLA抗体明确其在卵泡形成、卵母细胞发育及卵巢功能中的分子机制。
FIGLA (Folliculogenesis Specific BHLH Transcription Factor) is an oocyte-specific transcription factor critical for ovarian folliculogenesis and female fertility. It belongs to the basic helix-loop-helix (BHLH) protein family and plays a pivotal role in regulating the expression of zona pellucida (ZP) genes (e.g., ZP1. ZP2. ZP3), which are essential for forming the extracellular matrix surrounding oocytes. FIGLA is predominantly expressed during early oocyte development, where it promotes primordial follicle formation, maintains oocyte survival, and coordinates the transcriptional network required for follicular maturation. Dysregulation or mutations in FIGLA are linked to premature ovarian insufficiency (POI) and infertility.
FIGLA antibodies are valuable tools for studying oocyte biology, folliculogenesis, and reproductive disorders. They enable the detection and localization of FIGLA protein in ovarian tissues, aiding research on its expression patterns, regulatory mechanisms, and interactions with other folliculogenesis-related factors. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Understanding FIGLA's role through antibody-based assays contributes to insights into female reproductive health, genetic causes of infertility, and potential therapeutic targets for ovarian dysfunction. Commercial FIGLA antibodies are typically developed in rabbits or mice, with validation in model organisms like mice or humans.
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