Mouse Monoclonal ATG4D Antibody

The ATG4D antibody is a crucial tool for studying autophagy, a cellular degradation process essential for maintaining homeostasis. ATG4D, a member of the ATG4 cysteine protease family, plays a pivotal role in autophagosome formation by cleaving LC3/GABARAP proteins to enable their lipidation and subsequent membrane conjugation. Unlike other ATG4 isoforms (ATG4A-C), ATG4D exhibits distinct substrate preferences and regulatory mechanisms, with emerging roles in selective autophagy pathways and cellular stress responses. It is implicated in various physiological and pathological processes, including cancer progression, neurodegenerative diseases, and immune regulation. Antibodies targeting ATG4D are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and post-translational modifications. These antibodies help elucidate ATG4D’s interaction with autophagy-related proteins, its activation under nutrient-deprived conditions, and its crosstalk with pathways like mTOR and AMPK. Recent studies also explore ATG4D’s non-canonical functions, such as regulating mitochondrial quality control and apoptosis. Reliable ATG4D antibodies are vital for advancing research on autophagy dysregulation in diseases and developing therapeutic strategies targeting this pathway.