| WB | 1/1000 | Human,Mouse,zebrafish, |
| IF | 咨询技术 | Human,Mouse,zebrafish, |
| IHC | 咨询技术 | Human,Mouse,zebrafish, |
| ICC | 技术咨询 | Human,Mouse,zebrafish, |
| FCM | 咨询技术 | Human,Mouse,zebrafish, |
| Elisa | 咨询技术 | Human,Mouse,zebrafish, |
| Aliases | Cysteine protease ATG4B, 3422-, Autophagy-related protein 4 homolog B, atg4b, apg4b |
| WB Predicted band size | 44.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,zebrafish, |
| Immunogen | This DANRE atg4b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of DANRE atg4b. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **DANRE (Danio rerio) ATG4B (N-term) 抗体** 的3篇参考文献，简要概括了文献名称、作者和摘要内容：
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1. **文献名称**: *Autophagy regulates zebrafish larval heart regeneration by preventing senescence in regenerating cardiomyocytes*
**作者**: Li Y, et al.
**摘要**: 该研究利用斑马鱼模型探究自噬在心脏再生中的作用，发现ATG4B(通过N端特异性抗体检测)在再生心肌细胞中高表达，并通过清除受损细胞器维持细胞稳态，抑制细胞衰老，从而促进心脏再生。
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2. **文献名称**: *Characterization of autophagy-related genes in zebrafish embryos under oxidative stress*
**作者**: Zhang Q, et al.
**摘要**: 研究通过Western blot和免疫荧光(使用DANRE ATG4B N端抗体)分析了斑马鱼胚胎在氧化应激条件下自噬相关基因的表达变化，证实ATG4B在调控LC3蛋白加工及自噬体形成中起关键作用。
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3. **文献名称**: *Functional analysis of Atg4 paralogs in zebrafish development*
**作者**: Wang H, et al.
**摘要**: 本研究通过敲降和过表达实验，结合ATG4B N端特异性抗体的免疫组化验证，揭示了斑马鱼Atg4b在胚胎发育过程中通过调控自噬流影响神经管闭合和体节形成的分子机制。
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**备注**：以上文献为示例，实际文献需通过数据库(如PubMed、Google Scholar)检索关键词“Danio rerio ATG4B antibody”或联系抗体供应商(如Sigma-Aldrich、Abcam)获取产品引用的原始文献。
The DANRE ATG4B (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to detect the N-terminal region of autophagy-related protein 4B (ATG4B) in *Danio rerio* (zebrafish). ATG4B is a cysteine protease critical for autophagosome formation, a key step in autophagy—a conserved cellular process for degrading and recycling cytoplasmic components. ATG4B cleaves the C-terminal region of LC3/ATG8 family proteins (e.g., LC3B) to expose a glycine residue, enabling their conjugation to phosphatidylethanolamine on autophagosomal membranes. This processing is essential for autophagosome elongation and cargo recruitment.
The antibody targets the N-terminal epitope of zebrafish ATG4B, allowing researchers to study its expression, localization, and function in zebrafish models. Zebrafish are widely used for developmental biology and disease modeling due to their genetic tractability and transparency during early stages. By detecting ATG4B, this tool aids in investigating autophagy's role in zebrafish embryogenesis, organ development, or disease contexts like neurodegeneration or cancer. It is typically validated via Western blot, immunofluorescence, or immunohistochemistry, often with knockout controls to confirm specificity. Research using this antibody contributes to understanding conserved autophagy mechanisms and their implications in physiology and pathology, potentially informing therapeutic strategies targeting autophagy dysregulation.
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