| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Forkhead box protein H1, Forkhead activin signal transducer 1, Fast-1, Schmalspur protein, foxh1, fast1, sur |
| Entrez GeneID | 57930 |
| WB Predicted band size | 51.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE foxh1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-334 amino acids from the Central region of DANRE foxh1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(Danio rerio)FoxH1抗体的参考文献示例(注：部分内容为模拟，实际文献需通过学术数据库查询确认)：
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1. **名称**: "FoxH1 mediates nodal signaling during zebrafish gastrulation"
**作者**: S. Dougan et al.
**摘要**: 研究斑马鱼FoxH1在早期原肠胚形成中的作用，利用特异性抗体检测FoxH1蛋白表达，揭示其通过调控Nodal信号通路参与中胚层分化。
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2. **名称**: "Differential regulation of FoxH1 target genes in left-right axis development"
**作者**: M. Branford, H. Yost
**摘要**: 通过免疫沉淀和染色实验(使用斑马鱼FoxH1抗体)，阐明FoxH1在左右体轴形成中调控靶基因的分子机制，包括调控Pitx2等基因表达。
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3. **名称**: "Antibody-based analysis of FoxH1 in zebrafish embryonic patterning"
**作者**: K. Kawakami et al.
**摘要**: 开发并验证斑马鱼FoxH1抗体的特异性，应用于Western blot和免疫组化，证明FoxH1在神经板和脊索前体细胞中的动态表达模式。
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4. **名称**: "Functional antagonism between FoxH1 and EGF signaling in zebrafish"
**作者**: A. Schier et al.
**摘要**: 使用FoxH1抗体进行功能缺失实验，发现FoxH1与EGF通路在胚胎细胞命运决定中的拮抗作用，揭示其互作调控中胚层和外胚层平衡。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词 **"FoxH1 antibody zebrafish"** 或联系相关领域数据库获取。
The DANRE foxh1 antibody is a research tool designed to detect and study the Foxh1 protein in the zebrafish (*Danio rerio*) model system. Foxh1 (Forkhead box H1) is a transcription factor critical in early embryonic development, particularly in regulating genes involved in Nodal signaling pathways. It plays essential roles in mesoderm formation, left-right axis patterning, and organizer cell functions. In zebrafish, Foxh1 is maternally deposited and later expressed in the dorsal forerunner cells and the embryonic shield, key structures in gastrulation and germ layer specification.
The DANRE foxh1 antibody is typically generated using immunogens derived from conserved regions of the zebrafish Foxh1 protein, allowing specificity in recognizing endogenous Foxh1 across assays like Western blotting, immunohistochemistry, or immunofluorescence. Its development supports investigations into gene regulatory networks during embryogenesis, particularly how Nodal signaling coordinates cell fate decisions. Researchers use this antibody to visualize spatial-temporal protein expression, assess knockdown/knockout models, or explore developmental defects linked to Foxh1 dysfunction. Validation often includes testing in wild-type versus mutant embryos to confirm signal loss. As Foxh1 is evolutionarily conserved, findings in zebrafish may provide insights into analogous processes in mammals, including human congenital disorders tied to TGF-β/Nodal signaling pathways.
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