| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeodomain-interacting protein kinase 4, HIPK4 |
| Entrez GeneID | 147746 |
| WB Predicted band size | 69.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HIPK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-566 amino acids from the C-terminal region of human HIPK4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇涉及HIPK4抗体的参考文献概述(内容基于公开研究整理，非真实文献，仅供参考)：
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1. **文献名称**：*HIPK4 regulates spermatogenesis through kinase activity-dependent mechanisms in mice*
**作者**：Smith A, et al.
**摘要**：本研究利用HIPK4基因敲除小鼠模型，发现HIPK4缺失导致雄性不育。通过开发特异性HIPK4抗体，证实其在睾丸组织中的定位，揭示其通过磷酸化下游靶蛋白调控精子变形过程。
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2. **文献名称**：*Development of a monoclonal antibody against human HIPK4 for cancer biomarker studies*
**作者**：Zhang L, et al.
**摘要**：报道了一种新型抗人HIPK4单克隆抗体的制备与验证，通过免疫组化及Western blot验证其在多种癌细胞系中的特异性表达，提示HIPK4可能与肿瘤细胞增殖及耐药性相关。
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3. **文献名称**：*Structural insights into HIPK4 kinase domain and antibody interaction*
**作者**：Chen R, et al.
**摘要**：解析了HIPK4激酶结构域的三维结构，并利用抗体共结晶技术揭示其活性位点。研究为基于抗体的HIPK4功能抑制策略提供了分子基础，可能用于治疗相关生殖疾病。
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如需实际文献，建议通过PubMed或Google Scholar搜索关键词“HIPK4 antibody”或“HIPK4 kinase function”获取最新研究。
**Background of HIPK4 Antibody**
The **HIPK4 antibody** is a tool used to detect and study homeodomain-interacting protein kinase 4 (HIPK4), a serine/threonine kinase belonging to the HIPK family. HIPKs regulate transcription, cell proliferation, apoptosis, and DNA damage responses. HIPK4. though less characterized than HIPK1-3. is implicated in tissue-specific roles, particularly in spermatogenesis and neuronal development. It interacts with transcription factors and chromatin modifiers, modulating signaling pathways like Wnt and p53.
HIPK4 antibodies are essential for investigating its expression, localization, and function in cellular models. They are used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Studies suggest HIPK4 may influence sperm cell differentiation and neuronal connectivity, with dysregulation linked to infertility or neurodevelopmental disorders.
Structurally, HIPK4 contains a conserved kinase domain and unique N-terminal regions, which antibodies often target for specificity. Commercial HIPK4 antibodies are typically validated in knockout models to ensure minimal cross-reactivity. Researchers rely on these antibodies to explore HIPK4's role in development, disease, and potential therapeutic targeting.
When using HIPK4 antibodies, factors like tissue specificity, post-translational modifications, and proper controls are critical for accurate interpretation. Storage conditions (-20°C, avoiding freeze-thaw cycles) and validation in relevant experimental systems are recommended for optimal performance.
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