Rabbit Polyclonal GNAS Antibody

The GNAS antibody targets proteins encoded by the GNAS gene, a complex locus critical in G protein-coupled receptor (GPCR) signaling. GNAS encodes the α-subunit of the stimulatory G protein (Gsα), which activates adenylate cyclase to generate cAMP, regulating numerous cellular processes. The gene exhibits genomic imprinting, producing multiple transcripts (e.g., Gsα, XLαs, NESP55) through alternative promoters and splicing, with expression patterns dependent on parental origin. Mutations or dysregulation in GNAS are linked to disorders like McCune-Albright syndrome (activating mutations causing mosaicism), pseudohypoparathyroidism (PHP, due to maternal allele inactivation), and various tumors (e.g., pituitary adenomas, pancreatic carcinomas). GNAS antibodies are essential tools in research and diagnostics, enabling detection of protein expression, post-translational modifications (e.g., GTP-binding), and aberrant signaling in diseases. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Challenges include distinguishing between GNAS isoforms and accounting for tissue-specific imprinting effects. Reliable GNAS antibodies aid in understanding cAMP pathway dysregulation, tumorigenesis mechanisms, and developing targeted therapies for GNAS-related pathologies.