| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tax-responsive enhancer element B67; ATF4 protein |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-252 amino acids from human ATF4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇涉及ATF4 (S245)抗体的代表性文献摘要(注：S245磷酸化位点研究相对较少，以下为模拟示例，实际文献需根据具体数据库检索验证)：
---
1. **文献名称**: *"Phosphorylation of ATF4 at Serine 245 Mediates Cellular Stress Response in Neuronal Cells"*
**作者**: Lee et al., 2018
**摘要**: 本研究揭示了ATF4在S245位点的磷酸化在神经元氧化应激中的调控作用，通过特异性抗体证实了内质网应激条件下该位点的磷酸化增强，并促进下游CHOP蛋白的表达。
---
2. **文献名称**: *"A Novel Antibody-Based Detection of ATF4 Phosphorylation Marks Protein Misfolding in Alzheimer's Disease Models"*
**作者**: Rodriguez et al., 2020
**摘要**: 开发了一种针对ATF4 S245磷酸化位点的单克隆抗体，应用于阿尔茨海默病小鼠模型的脑组织分析，证明该位点磷酸化水平与tau蛋白异常聚集正相关。
---
3. **文献名称**: *"ATF4 Ser245 Phosphorylation Regulates Autophagy-Lysosomal Pathway in Cancer Chemoresistance"*
**作者**: Chen & Wei, 2021
**摘要**: 利用ATF4 (S245)抗体发现化疗药物诱导的S245磷酸化通过激活自噬通路增强癌细胞存活，提示该位点可作为耐药性治疗的潜在靶点。
---
4. **文献名称**: *"Validation of a Phospho-Specific ATF4 (Ser245) Antibody for Studying ER Stress in Human Hepatocytes"*
**作者**: Gupta et al., 2019
**摘要**: 验证了ATF4 S245磷酸化抗体在人类肝细胞中的特异性，证明其在衣霉素诱导的内质网应激模型中可靠检测ATF4激活状态，并与肝纤维化标志物相关。
---
**备注**：以上文献为示例性质，实际研究中需根据抗体生产商(如CST、Abcam)提供的产品引用文献或通过PubMed精准检索。建议结合具体实验背景筛选最新论文。
The ATF4 (S245) antibody is designed to specifically detect the phosphorylation of activating transcription factor 4 (ATF4) at serine 245. a post-translational modification critical for regulating its activity. ATF4. a member of the CREB/ATF family of basic leucine zipper (bZIP) transcription factors, plays a central role in cellular stress responses, including the integrated stress response (ISR), endoplasmic reticulum (ER) stress, and amino acid deprivation. Phosphorylation at Ser245. mediated by kinases such as casein kinase 1 (CK1) or CK2. enhances ATF4 stability and transcriptional activity, enabling it to regulate genes involved in adaptation to stress, redox homeostasis, and apoptosis (e.g., CHOP, ASNS).
This antibody is commonly used in research to study ATF4 activation dynamics under stress conditions, such as nutrient deprivation, hypoxia, or ER stress, and its dysregulation in diseases like cancer, neurodegenerative disorders, and metabolic syndromes. Validated for applications like Western blotting, immunofluorescence, and immunoprecipitation, the ATF4 (S245) antibody helps elucidate phosphorylation-dependent mechanisms of ATF4 signaling. Specificity is often confirmed using knockout models or phosphatase-treated lysates. Its utility spans molecular biology, cancer biology, and translational studies exploring stress-response pathways and therapeutic targets.
×
