Rabbit Polyclonal COXIV Antibody

The cytochrome c oxidase subunit IV (COXIV) antibody is a key tool in mitochondrial research, targeting a nuclear-encoded subunit of the cytochrome c oxidase (COX) complex (Complex IV) in the mitochondrial electron transport chain. COXIV is embedded in the inner mitochondrial membrane and plays a critical role in cellular energy production by facilitating the transfer of electrons to oxygen during oxidative phosphorylation. As a housekeeping protein with stable expression across tissues and metabolic conditions, COXIV is widely used as a loading control in mitochondrial studies, particularly when analyzing mitochondrial content or comparing mitochondrial fractions in Western blotting, immunofluorescence, or immunohistochemistry. Its specificity for the mitochondrial compartment makes it valuable for verifying mitochondrial integrity in experiments involving mitochondrial isolation or stress-induced damage. Antibodies against COXIV are often raised in rabbits or mice and validated for cross-reactivity in human, rat, and mouse samples. Researchers apply COXIV antibodies in diverse fields, including cancer metabolism, neurodegenerative diseases, and aging studies, where mitochondrial dysfunction is a focal point. Proper validation ensures minimal cross-reactivity with non-mitochondrial proteins, reinforcing its reliability as a reference marker.