Rabbit Polyclonal betaIITubulin Antibody

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     All lanes : Anti-beta II Tubulin at 1:8000 dilution Lane 1: mouse brain lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: PC-12 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (mouse myoblast cell line) cells labeling beta II Tubulin with P34170 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG  secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on C2C12 cell line. The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing NIH/3T3 cells stained with P34170 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34170,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

Beta II Tubulin (βIII-Tubulin), a member of the tubulin protein family, is a cytoskeletal component essential for microtubule formation. Microtubules play critical roles in cell structure, intracellular transport, and mitosis. Unlike the ubiquitously expressed beta I Tubulin, beta II Tubulin (encoded by the TUBB3 gene) is neuron-specific and serves as a marker for differentiating neurons during development. Its expression is particularly prominent in postmitotic neurons, where it contributes to axonal growth, synaptic plasticity, and neurotransmitter release.

Antibodies targeting beta II Tubulin are widely used in neuroscience and cancer research. In neurobiology, they help identify neuronal populations, track neurogenesis, and study neurodegenerative diseases like Alzheimer's. In oncology, beta II Tubulin overexpression correlates with aggressive tumors and resistance to microtubule-targeting chemotherapeutics (e.g., taxanes), making it a prognostic biomarker. These antibodies are also employed to assess mitotic activity in dividing cells.

Commercially available beta II Tubulin antibodies are typically validated for techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Specificity is crucial, as cross-reactivity with other beta-tubulin isoforms (e.g., beta I or beta IV) can occur depending on epitope recognition. Some studies use beta II Tubulin antibodies as loading controls in neuronal tissues due to its stable expression. Ongoing research explores its role in cellular stress responses and interactions with microtubule-associated proteins (MAPs), further expanding its biomedical relevance.