| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Beta-crystallin B2, Beta-B2 crystallin, Beta-crystallin Bp, CRYBB2, CRYB2, CRYB2A |
| Entrez GeneID | 1415 |
| WB Predicted band size | 23.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This CRYBB2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4-36 amino acids from the N-terminal region of human CRYBB2. |
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以下是关于CRYBB2 (N-term)抗体的3篇参考文献示例(注：部分内容为模拟，实际文献需根据数据库查询确认)：
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1. **标题**: "A novel mutation in CRYBB2 associated with autosomal dominant congenital cataract disrupts N-terminal protein stability"
**作者**: Zhang et al.
**摘要**: 研究通过全外显子测序发现CRYBB2的N端错义突变(p.Pro24Leu)导致先天性白内障。利用抗CRYBB2 N端抗体进行Western blot和免疫荧光，证实突变体蛋白在晶状体上皮细胞中异常聚集，稳定性下降。
2. **标题**: "Developmental regulation of βB2-crystallin in murine lens: Insights from CRYBB2 N-terminal specific antibody"
**作者**: Kumar et al.
**摘要**: 研究使用CRYBB2 N端特异性抗体分析小鼠胚胎至成体晶状体的蛋白表达。免疫组化显示CRYBB2在胚胎发育早期广泛分布，成年后局限于晶状体核，提示其与晶状体纤维细胞分化的相关性。
3. **标题**: "CRYBB2 N-terminal acetylation modulates lens transparency: Evidence from antibody-based proteomic profiling"
**作者**: Li et al.
**摘要**: 通过CRYBB2 N端抗体结合质谱技术，发现N端乙酰化修饰在维持晶状体透明度中的作用。研究揭示乙酰化缺失导致CRYBB2与其他晶状体蛋白相互作用异常，可能参与白内障发生。
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**备注**：以上文献为示例，实际引用时建议通过PubMed、Google Scholar等平台以关键词“CRYBB2 antibody N-terminal”或“CRYBB2 N-term epitope”检索，并筛选方法学中明确使用该抗体的研究。部分厂商(如Thermo Fisher、Abcam)的产品说明书也可能提供相关参考文献。
The CRYBB2 (N-term) antibody is a specialized tool designed to detect the N-terminal region of the β-crystallin B2 (CRYBB2) protein, a member of the βγ-crystallin superfamily. CRYBB2 is predominantly expressed in the lens of the eye and plays a critical role in maintaining lens transparency and refractive index by forming stable, soluble structural proteins. Mutations in the CRYBB2 gene are associated with congenital cataracts and other lens-related disorders, making this protein a focus in ophthalmology research.
The antibody targets epitopes near the N-terminus of CRYBB2. which is involved in protein-protein interactions and structural organization within lens fiber cells. It is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study CRYBB2 expression, localization, and post-translational modifications in both normal and pathological contexts. Researchers also employ it to investigate cataractogenesis mechanisms or validate CRYBB2 knockout/knockdown models.
As a rabbit or mouse polyclonal/monoclonal antibody, its specificity is validated through controls like peptide-blocking assays. Commercial variants often include citations linking CRYBB2 dysfunction to autosomal dominant cataracts, emphasizing its clinical relevance. Proper storage (typically at -20°C) and optimized dilution protocols are essential for maintaining reactivity. This tool bridges structural biology, developmental studies, and disease modeling in ocular research.
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