| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | THO complex subunit 6 homolog, Functional spliceosome-associated protein 35, fSAP35, WD repeat-containing protein 58, THOC6, WDR58 |
| Entrez GeneID | 79228 |
| WB Predicted band size | 37.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This THOC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-123 amino acids from the Central region of human THOC6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于THOC6抗体的参考文献示例(注：部分内容为虚构示例，仅用于演示格式)：
1. **文献名称**：*THOC6 mutations cause syndromic intellectual disability through disrupted mRNA export*
**作者**：Smith A, et al.
**摘要**：本研究揭示了THOC6基因突变通过破坏mRNA输出导致神经发育障碍的机制。通过使用特异性THOC6抗体进行免疫印迹和免疫荧光实验，发现患者细胞中THOC6蛋白表达显著降低，且其与THO复合体其他成员的相互作用受损。
2. **文献名称**：*Characterization of a novel monoclonal antibody against THOC6 for cancer research*
**作者**：Chen L, et al.
**摘要**：研究团队开发了一种新型THOC6单克隆抗体，并验证其在流式细胞术和免疫组化中的应用。实验表明，该抗体可特异性识别多种癌细胞系中的THOC6蛋白，为研究其在白血病中的异常表达提供了工具。
3. **文献名称**：*THOC6 deficiency disrupts embryonic development via impaired RNA processing*
**作者**：Wang Y, et al.
**摘要**：通过在小鼠模型中结合CRISPR敲除和THOC6抗体的蛋白质定位分析，本研究证明THOC6缺失导致胚胎致死，其机制与RNA加工异常及p53通路激活相关。
4. **文献名称**：*THOC6 antibody-based proteomic profiling in genetic syndromes*
**作者**：Johnson R, et al.
**摘要**：利用THOC6抗体对Beaulieu-Boycott-Innes综合征患者样本进行蛋白质组学分析，发现THOC6与其他转录调控蛋白共定位，提示其在基因表达调控中的关键作用。
(注：以上文献为示例，实际引用请查询真实数据库。)
The THOC6 antibody is a valuable tool in molecular biology research, targeting the THOC6 protein, a component of the THO complex. This evolutionarily conserved multiprotein complex is essential for mRNA processing, export, and genome stability. THOC6 specifically interacts with other THO subcomplex components (THOC1-THOC7) and plays a critical role in coordinating transcriptional elongation with nuclear mRNA export. Studies link THOC6 mutations to neurodevelopmental disorders, particularly Xia-Gibbs syndrome, characterized by intellectual disability, delayed development, and distinct facial features.
As a reagent, THOC6 antibody enables detection and quantification of THOC6 expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Its applications span investigations into mRNA metabolism, gene expression regulation, and disease mechanisms. Researchers utilize it to explore THOC6's tissue-specific expression patterns, subcellular localization, and interactions within the THO/TREX complex. Commercial THOC6 antibodies are typically raised in rabbits or mice, with validation in knockout cell lines to ensure specificity. Recent studies also implicate THOC6 in cancer progression and cellular stress responses, expanding its relevance in translational research. This antibody remains crucial for dissecting THOC6's dual roles in fundamental cellular processes and human pathologies.
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