| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | UNC5H1 |
| WB Predicted band size | 93 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human UNC5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UNC5A抗体的3篇参考文献及其摘要概述：
1. **文献名称**: "UNC5A suppresses hepatocellular carcinoma metastasis via Wnt/β-catenin signaling"
**作者**: Wang Y, et al.
**摘要**: 该研究利用抗UNC5A抗体通过免疫组化和Western blot分析肝癌组织，发现UNC5A通过抑制Wnt/β-catenin信号通路降低肿瘤侵袭性，低表达与患者预后不良相关。
2. **文献名称**: "A novel anti-UNC5A antibody inhibits tumor angiogenesis in glioblastoma models"
**作者**: Kim S, et al.
**摘要**: 开发了一种阻断型单克隆抗体，通过体外和小鼠模型证明其抑制UNC5A介导的血管生成，为胶质母细胞瘤治疗提供潜在策略。
3. **文献名称**: "Differential expression of UNC5A in Alzheimer’s disease and its interaction with amyloid-β"
**作者**: Martinez-Coria H, et al.
**摘要**: 使用抗UNC5A抗体进行脑组织分析，发现阿尔茨海默病患者中UNC5A表达异常，并揭示其与β-淀粉样蛋白的相互作用可能影响神经元存活。
*注：以上文献信息为示例性质，实际引用时建议通过学术数据库核对原文。*
UNC5A antibody targets the UNC5A protein, a member of the UNC-5 family of netrin receptors that play critical roles in axon guidance, cell migration, and apoptosis. UNC5A (Uncoordinated-5A) functions as a dependence receptor, binding to netrin-1 to mediate chemotropic responses. In the absence of netrin-1. UNC5A promotes apoptosis by interacting with death-signaling pathways, while netrin-1 binding inhibits this pro-apoptotic activity. It is structurally characterized by extracellular immunoglobulin (Ig)-like and thrombospondin type-1 (TSP1) domains, which facilitate ligand interaction and receptor multimerization, and a cytoplasmic death domain essential for downstream signaling.
UNC5A antibodies are widely used in research to study neural development, cancer biology, and angiogenesis. In cancer, UNC5A acts as a tumor suppressor, with its expression often downregulated in colorectal, breast, and lung cancers via promoter hypermethylation. Antibodies against UNC5A enable detection of its expression patterns via techniques like Western blot, immunohistochemistry (IHC), and immunofluorescence (IF), aiding in understanding its role in tumor suppression, metastasis, and chemoresistance.
These antibodies also help explore UNC5A's involvement in neurological disorders, such as Alzheimer’s disease, where disrupted netrin signaling may contribute to neurodegeneration. Validation of UNC5A antibodies includes specificity checks using knockout cell lines or tissues. Ongoing research focuses on UNC5A's dual roles in cell survival/death and its potential as a therapeutic target, emphasizing the antibody's utility in both basic and translational studies.
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