| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MEM3; PARK17 |
| WB Predicted band size | 92 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human VPS35 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于VPS35抗体的3篇参考文献及其简要摘要内容：
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1. **文献名称**：*VPS35 mutations in Parkinson disease*
**作者**：Vilariño-Güell C, Wider C, Ross OA, et al.
**摘要**：该研究首次报道了VPS35基因突变与家族性帕金森病的关联。通过免疫印迹(Western blot)和免疫组化实验，发现突变导致VPS35蛋白表达异常，影响逆转录酶复合体的功能，进而破坏细胞内囊泡运输机制。
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2. **文献名称**：*Retromer deficiency observed in Alzheimer’s disease causes hippocampal dysfunction*
**作者**：Small SA, Kent K, Pierce A, et al.
**摘要**：研究探讨了阿尔茨海默病中VPS35/逆转录酶复合体的功能缺陷。利用特异性抗体检测发现，患者脑组织中VPS35蛋白水平显著降低，导致神经元内淀粉样前体蛋白(APP)运输障碍，可能与病理蛋白沉积相关。
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3. **文献名称**：*VPS35 regulates tau phosphorylation and synaptic function*
**作者**：Wen L, Tang FL, Hong Y, et al.
**摘要**：该研究通过免疫荧光和共聚焦显微镜技术，验证了VPS35抗体在小鼠脑组织中的特异性定位。结果发现VPS35缺失会增强tau蛋白过度磷酸化，破坏突触功能，提示其与tau蛋白病的潜在联系。
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**备注**：上述文献均涉及VPS35抗体在疾病模型或临床样本中的应用(如蛋白表达分析、定位研究等)。若需具体实验细节或更早期研究，建议进一步查阅方法学论文或抗体开发相关文献。
VPS35 (Vacuolar Protein Sorting 35 Homolog) is a critical component of the retromer complex, a conserved protein assembly responsible for orchestrating endosome-to-Golgi retrograde transport of cargo proteins. This process is essential for maintaining cellular homeostasis, enabling the recycling of receptors, transporters, and enzymes, thereby regulating diverse physiological functions such as lysosomal degradation, autophagy, and signaling pathways. Dysregulation of VPS35 has been implicated in neurodegenerative diseases, particularly Parkinson’s disease, where specific mutations (e.g., D620N) disrupt retromer function, leading to pathological protein aggregation and neuronal death.
VPS35 antibodies are vital tools for studying the expression, localization, and function of VPS35 in both physiological and disease contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cultured cells. High-quality VPS35 antibodies are rigorously validated for specificity, often using knockout cell lines or tissues to confirm minimal cross-reactivity. Researchers also employ them to investigate interactions within the retromer complex (e.g., with VPS26 and VPS29) or to explore disease mechanisms in models of neurodegeneration, cancer, or metabolic disorders. The development of phospho-specific or mutation-targeting antibodies further aids in dissecting post-translational modifications or pathogenic variants. As therapeutic strategies targeting retromer dysfunction emerge, VPS35 antibodies remain indispensable for preclinical research and biomarker discovery.
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