| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PYHIN2; IFNGIP1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human IFI16 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IFI16抗体的3篇代表性文献摘要概述：
1. **《IFI16 is an innate immune sensor for intracellular DNA》**
- Authors: Unterholzner L, et al.
- 摘要：该研究首次证实IFI16作为细胞内核DNA识别蛋白，通过STING信号通路激活干扰素反应，揭示了其在抗病毒免疫中的关键作用。研究使用特异性抗体验证了IFI16与病毒DNA的相互作用。
2. **《Autoantibodies to the interferon-inducible IFI16 protein in systemic lupus erythematosus》**
- Authors: Mondini M, et al.
- 摘要：研究发现系统性红斑狼疮(SLE)患者血清中存在抗IFI16自身抗体，提示IFI16可能参与SLE的自身免疫反应机制。研究通过ELISA和免疫印迹法检测抗体水平。
3. **《The interferon-inducible protein IFI16 recognizes herpesvirus capsids by its HIN domains》**
- Authors: Kerur N, et al.
- 摘要：本文阐明了IFI16通过HIN结构域直接结合疱疹病毒衣壳蛋白，介导抗病毒天然免疫应答。实验利用IFI16抗体进行免疫共沉淀和免疫荧光定位分析。
4. **《IFI16 promotes prostate cancer progression by activating the AKT/mTOR pathway》**
- Authors: Alimirah F, et al.
- 摘要：研究显示IFI16在前列腺癌中高表达，并通过AKT/mTOR通路促进肿瘤生长。使用特异性抗体通过免疫组化验证了IFI16在癌组织中的表达上调。
这些文献覆盖了IFI16在先天免疫、自身免疫病、抗病毒机制及癌症中的功能研究，涉及抗体在机制验证、诊断标志物及治疗靶点探索中的应用。
IFI16 (Interferon Gamma Inducible Protein 16) is a member of the PYHIN protein family, primarily involved in innate immune responses. It functions as a nuclear DNA sensor, detecting pathogenic or mislocalized DNA from viruses, bacteria, or damaged cells, thereby triggering interferon and pro-inflammatory cytokine production. Structurally, IFI16 contains two HIN-200 domains for DNA binding and a pyrin domain for protein-protein interactions.
Research highlights its dual role in antiviral defense and autoimmunity. It restricts viral replication (e.g., HSV, HPV) by modulating transcription or promoting chromatinization. However, aberrant IFI16 activity is linked to autoimmune diseases like systemic lupus erythematosus (SLE) and Sjögren’s syndrome, where autoantibodies against IFI16 correlate with disease severity.
IFI16 antibodies are essential tools for studying its expression, localization, and interactions in immune signaling pathways. They are used in techniques like Western blot, immunofluorescence, and immunohistochemistry to explore IFI16's role in infection, cancer (e.g., suppressing oncogenic viruses or influencing tumor progression), and autoimmune mechanisms. Dysregulated IFI16 expression in cancers also positions it as a potential diagnostic or therapeutic target.
Overall, IFI16 antibodies provide critical insights into its multifaceted functions in immunity and disease, bridging basic research and clinical applications.
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